Product: MRFAP1 Antibody
Catalog: DF12424
Description: Rabbit polyclonal antibody to MRFAP1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Bovine, Dog
Mol.Wt.: 15 kDa; 15kD(Calculated).
Uniprot: Q9Y605
RRID: AB_2845229

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MRFAP1 Antibody detects endogenous levels of total MRFAP1.
RRID:
AB_2845229
Cite Format: Affinity Biosciences Cat# DF12424, RRID:AB_2845229.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Mof4 family associated protein 1; MOFA1_HUMAN; MORF4 family associated protein 1; MORF4 family-associated protein 1; Mrfap1; OTTHUMP00000155159; OTTHUMP00000217763; PAM14; PGR1; Protein associated with MRG 14 kDa; Protein associated with MRG of 14 kDa; Protein PGR1; T cell activation protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MRPLDIVELAEPEEVEVLEPEEDFEQFLLPVINEMREDIASLTREHGRAYLRNRSKLWEMDNMLIQIKTQVEASEESALNHLQNPGDAAEGRAAKRCEKAEEKAKEIAKMAEMLVELVRRIEKSESS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
83
Horse
75
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y605 As Substrate

Site PTM Type Enzyme
S55 Phosphorylation
K56 Ubiquitination
K68 Ubiquitination
K95 Ubiquitination
K109 Ubiquitination
K123 Ubiquitination

Research Backgrounds

Subcellular Location:

Nucleus. Cytoplasm>Perinuclear region.
Note: Colocalizes with MORF4L1 to cell nuclei.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex composed of MORF4L1, MRFAP1 and RB1. Interacts via its N-terminus with MORF4L1. Interacts with CSTB and MORF4L2.

Family&Domains:

Belongs to the MORF4 family-associated protein family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.