NAT10 Antibody - #DF12427

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Product: NAT10 Antibody
Catalog: DF12427
Description: Rabbit polyclonal antibody to NAT10
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 116 kDa; 116kD(Calculated).
Uniprot: Q9H0A0
RRID: AB_2845232

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NAT10 Antibody detects endogenous levels of total NAT10.
RRID:
AB_2845232
Cite Format: Affinity Biosciences Cat# DF12427, RRID:AB_2845232.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; hALP; KIAA1709; N acetyltransferase 10; N acetyltransferase 10 GCN5 related; N acetyltransferase like; N acetyltransferase like protein; N-acetyltransferase 10; NAT10; NAT10_HUMAN; NET43;

Immunogens

Immunogen:

A synthesized peptide derived from human NAT10, corresponding to a region within the internal amino acids.

Uniprot:

Q9H0A0(NAT10_HUMAN) >>Visit HPA database.

Gene(ID):
NAT10(55226)
Sequence:
MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLYEVSLQESIRYAPGDAVEKWLNDLLCLDCLNITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA. May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. The relevance of the protein lysine acetyltransferase activity is however unsure in vivo. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization.

PTMs:

Acetylation at Lys-426 is required to activation of rRNA transcription. May be autoacetylated; however ability to autoacetylate in vivo requires additional evidences.

Subcellular Location:

Nucleus>Nucleolus. Midbody.
Note: Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

References

1). NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma. Acta Pharmaceutica Sinica B, 2022 (PubMed: 35967285) [IF=14.7]

Application: IHC    Species: Human    Sample:

Figure 1 Elevated NAT10 expression is associated with poor outcomes in MM patients. (A) NAT10 mRNA levels were significantly increased in MM samples. The signal level of NAT10 (217884_at signaling) was shown on the y-axis (NP, n = 22; MGUS, n = 44; MM, n = 351). (B) IHC staining verified that NAT10 protein level was elevated in MM patient samples and NAT10 expression was positively correlated with Ki67 expression in MM samples (NP, n = 10; MM, n = 20). (C–F) High expression of NAT10 indicted poor overall survival (OS) in TT2 (C), APEX (D), TT3 (E), and HOVON65 (F) cohorts. All data are displayed as mean ± SD;

Application: WB    Species: Human    Sample:

Figure 1 Elevated NAT10 expression is associated with poor outcomes in MM patients. (A) NAT10 mRNA levels were significantly increased in MM samples. The signal level of NAT10 (217884_at signaling) was shown on the y-axis (NP, n = 22; MGUS, n = 44; MM, n = 351). (B) IHC staining verified that NAT10 protein level was elevated in MM patient samples and NAT10 expression was positively correlated with Ki67 expression in MM samples (NP, n = 10; MM, n = 20). (C–F) High expression of NAT10 indicted poor overall survival (OS) in TT2 (C), APEX (D), TT3 (E), and HOVON65 (F) cohorts. All data are displayed as mean ± SD;

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