OPA3 Antibody - #DF12436

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Product: OPA3 Antibody
Catalog: DF12436
Description: Rabbit polyclonal antibody to OPA3
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Rabbit
Mol.Wt.: 20 kDa; 20kD(Calculated).
Uniprot: Q9H6K4
RRID: AB_2845241

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(83%), Bovine(92%), Horse(91%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
OPA3 Antibody detects endogenous levels of total OPA3.
RRID:
AB_2845241
Cite Format: Affinity Biosciences Cat# DF12436, RRID:AB_2845241.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ22187; FLJ25932; Iraqi Jewish optic atrophy plus (3 methylglutaconicaciduria type 3); MGA3; MGC75494; OPA 3; OPA3 protein; Optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia); Optic atrophy 3 (Iraqi Jewish 'optic atrophy plus'); Optic atrophy 3; Optic atrophy 3 protein;

Immunogens

Immunogen:
Uniprot:

Q9H6K4(OPA3_HUMAN) >>Visit HPA database.

Gene(ID):
OPA3(80207)
Expression:
Q9H6K4 OPA3_HUMAN:

Ubiquitous. Most prominent expression in skeletal muscle and kidney.

Sequence:
MVVGAFPMAKLLYLGIRQVSKPLANRIKEAARRSEFFKTYICLPPAQLYHWVEMRTKMRIMGFRGTVIKPLNEEAAAELGAELLGEATIFIVGGGCLVLEYWRHQAQQRHKEEEQRAAWNALRDEVGHLALALEALQAQVQAAPPQGALEELRTELQEVRAQLCNPGRSASHAVPASKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Horse
91
Pig
83
Rabbit
80
Sheep
60
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H6K4 As Substrate

Site PTM Type Enzyme
K57 Acetylation

Research Backgrounds

Function:

May play some role in mitochondrial processes.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Most prominent expression in skeletal muscle and kidney.

Family&Domains:

Belongs to the OPA3 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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