PHF19 Antibody - #DF12452
| Product: | PHF19 Antibody |
| Catalog: | DF12452 |
| Description: | Rabbit polyclonal antibody to PHF19 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken |
| Mol.Wt.: | 66 kDa; 66kD(Calculated). |
| Uniprot: | Q5T6S3 |
| RRID: | AB_2845257 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12452, RRID:AB_2845257.
Fold/Unfold
hPCL3; MGC131698; MGC149712; MGC149713; MGC23929; MTF2L1; OTTHUMP00000021996; OTTHUMP00000021998; PCL3; PHD finger protein 19; Phf19; PHF19 protein; PHF19_HUMAN; Polycomb like 3; Polycomb like protein 3; Polycomb-like protein 3; TDRD19B; Tudor domain containing 19B;
Immunogens
Isoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines.
- Q5T6S3 PHF19_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENRALDPGTRDSYGATSHLPNKGALAKVKNNFKDLMSKLTEGQYVLCRWTDGLYYLGKIKRVSSSKQSCLVTFEDNSKYWVLWKDIQHAGVPGEEPKCNICLGKTSGPLNEILICGKCGLGYHQQCHIPIAGSADQPLLTPWFCRRCIFALAVRKGGALKKGAIARTLQAVKMVLSYQPEELEWDSPHRTNQQQCYCYCGGPGEWYLRMLQCYRCRQWFHEACTQCLNEPMMFGDRFYLFFCSVCNQGPEYIERLPLRWVDVVHLALYNLGVQSKKKYFDFEEILAFVNHHWELLQLGKLTSTPVTDRGPHLLNALNSYKSRFLCGKEIKKKKCIFRLRIRVPPNPPGKLLPDKGLLPNENSASSELRKRGKSKPGLLPHEFQQQKRRVYRRKRSKFLLEDAIPSSDFTSAWSTNHHLASIFDFTLDEIQSLKSASSGQTFFSDVDSTDAASTSGSASTSLSYDSRWTVGSRKRKLAAKAYMPLRAKRWAAELDGRCPSDSSAEGASVPERPDEGIDSHTFESISEDDSSLSHLKSSITNYFGAAGRLACGEKYQVLARRVTPEGKVQYLVEWEGTTPY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5T6S3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| Y14 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| Y56 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K162 | Methylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K476 | Methylation | Uniprot | |
| K554 | Ubiquitination | Uniprot |
Research Backgrounds
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
Nucleus.
Isoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines.
Associated component of the PRC2 complex. Interacts with EZH2 (via its Tudor domain). Isoform 1 interacts with SUZ12; isoform 2 does not interact with SUZ12. Interacts with RIOX1.
The Tudor domain recognizes and binds H3K36me3 (PubMed:23273982, PubMed:23160351, PubMed:23104054 and PubMed:23228662). May also bind H3K27me3, with a lower affinity (PubMed:23160351).
Belongs to the Polycomblike family.
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