RAB12 Antibody | Affinity Biosciences

WB
Cites

1/2

RAB12 Antibody - Western blot analysis of extracts from B16F10, using RAB12 Antibody.

Product:	RAB12 Antibody
Catalog:	DF12459
Description:	Rabbit polyclonal antibody to RAB12
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	28 kDa; 27kD(Calculated).
Uniprot:	Q6IQ22
RRID:	AB_2845264

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

RAB12 Antibody detects endogenous levels of total RAB12.

RRID:

AB_2845264
Cite Format: Affinity Biosciences Cat# DF12459, RRID:AB_2845264.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Putative Ras related protein Rab 12; RAB12; RAB12, member RAS oncogene family; RAB12_HUMAN; Ras-related protein Rab-12;

Immunogens

Immunogen:

Uniprot:

Q6IQ22(RAB12_HUMAN) >>Visit HPA database.

Gene(ID):

RAB12(201475)

Sequence:

Q6IQ22 RAB12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPGAALQRRAGGGGGLGAGSPALSGGQGRRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGVDFKIKTVELRGKKIRLQIWDTAGQERFNSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREITRQQGEKFAQQITGMRFCEASAKDNFNVDEIFLKLVDDILKKMPLDILRNELSNSILSLQPEPEIPPELPPPRPHVRCC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6IQ22 As Substrate

Q6IQ22

Site	PTM Type	Source
M1	Acetylation	Uniprot
R10	Methylation	Uniprot
S21	Phosphorylation	Uniprot
S25	Phosphorylation	Uniprot
T83	Phosphorylation	Uniprot
T98	Phosphorylation	Uniprot
S106	Phosphorylation	Uniprot
T108	Phosphorylation	Uniprot
S109	Phosphorylation	Uniprot
K156	Ubiquitination	Uniprot
K172	Ubiquitination	Uniprot
S220	Phosphorylation	Uniprot

Research Backgrounds

Q6IQ22_RAB12_HUMAN

Function:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).

PTMs:

Phosphorylation of Ser-106 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

Subcellular Location:

Recycling endosome membrane>Lipid-anchor>Cytoplasmic side. Lysosome membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane. Cytoplasmic vesicle>Autophagosome.

Subunit Structure:

Interacts with RABIF. Interacts with OPTN (By similarity). Interacts with LRRK2; interaction facilitates phosphorylation of Ser-106. Interacts with GDI1, GDI2, CHM and CHML; these interactions are disrupted by phosphorylation on Ser-106. Interacts with RILPL1 and RILPL2; these interactions are dependent on phosphorylation of Ser-106.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

References

1). TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence, 2020 (PubMed: 32420802) [IF=5.5]

Application: WB Species: Mouse Sample: BSR-T7/5 cells

Figure 8. rSS1GFP infection affects the expression of cellular translation, posttranslational modification and trafficking-associated proteins. (A) The heatmap of representative 20 DEPs related to “Translation, ribosomal structure and biogenesis” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Translation, ribosomal structure and biogenesis” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “Posttranslational modification, protein turnover, chaperones” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “Posttranslational modification, protein turnover, chaperones” are analyzed by the STRING software. (E) The heatmap of representative 20 DEPs related to “Intracellular trafficking, secretion, and vesicular transport” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (F) The protein-protein interactions of the DEPs related to “Intracellular trafficking, secretion, and vesicular transport” are analyzed by the STRING software. (G) The mRNA expression levels of six selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (H) The protein expression levels of six DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of six DEPs were compared with the control GAPDH expression.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

RAB12 Antibody - #DF12459