Product: RBBP9 Antibody
Catalog: DF12462
Description: Rabbit polyclonal antibody to RBBP9
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 22 kDa; 21kD(Calculated).
Uniprot: O75884
RRID: AB_2845267

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(83%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
RBBP9 Antibody detects endogenous levels of total RBBP9.
RRID:
AB_2845267
Cite Format: Affinity Biosciences Cat# DF12462, RRID:AB_2845267.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B5T overexpressed gene protein; B5T-overexpressed gene protein; BOG; Bog protein; MGC9236; OTTHUMP00000030360; Protein BOG; Putative hydrolase RBBP9; RBBP 10; RBBP 9; RBBP-10; RBBP-9; RBBP10; RBBP9; RBBP9_HUMAN; Retinoblastoma binding protein 10; Retinoblastoma binding protein 9; Retinoblastoma-binding protein 10; Retinoblastoma-binding protein 9; Retinoma binding protein 9;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O75884 RBBP9_HUMAN:

Expressed at higher levels in tumor tissues such as carcinoma.

Sequence:
MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
92
Dog
92
Rabbit
92
Bovine
83
Xenopus
83
Zebrafish
82
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75884 As Substrate

Site PTM Type Enzyme
S76 Phosphorylation
R152 Methylation
K156 Ubiquitination

Research Backgrounds

Function:

Serine hydrolase whose substrates have not been identified yet. May negatively regulate basal or autocrine TGF-beta signaling by suppressing SMAD2-SMAD3 phosphorylation. May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta through interaction with RB1 and the subsequent displacement of E2F1.

Tissue Specificity:

Expressed at higher levels in tumor tissues such as carcinoma.

Subunit Structure:

Interacts with RB1; the interaction disrupts RB1 binding to E2F1. Interacts with RBL1 and RBL2 (By similarity).

Family&Domains:

Belongs to the RBBP9 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.