SMYD3 Antibody - #DF12477
| Product: | SMYD3 Antibody |
| Catalog: | DF12477 |
| Description: | Rabbit polyclonal antibody to SMYD3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine |
| Mol.Wt.: | 42-45 kDa; 49kD(Calculated). |
| Uniprot: | Q9H7B4 |
| RRID: | AB_2845282 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12477, RRID:AB_2845282.
Fold/Unfold
bA74P14.1 (novel protein); bA74P14.1; FLJ21080; histone lysine N methyltransferase SMYD3; KMT3E; MGC104324; SET and MYND domain containing 3; SET and MYND domain containing protein 3; SET and MYND domain-containing protein 3; SMYD 3; Smyd3; SMYD3 protein; SMYD3_HUMAN; Zinc finger MYND domain containing 1; Zinc finger MYND domain containing protein 1; Zinc finger MYND domain-containing protein 1; Zinc finger protein subfamily 3A MYND domain containing 1; Zinc finger protein, subfamily 3A (MYND domain containing), 1; ZMYND 1; ZMYND1; ZNFN3A1;
Immunogens
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
- Q9H7B4 SMYD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPLKVEKFATAKRGNGLRAVTPLRPGELLFRSDPLAYTVCKGSRGVVCDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKRECKCLKSCKPRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHWKWEQVLAMCQAIISSNSERLPDINIYQLKVLDCAMDACINLGLLEEALFYGTRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H7B4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K391 | Ubiquitination | Uniprot |
Research Backgrounds
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Cytoplasm. Nucleus.
Note: Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Interacts with HSPCA. Interacts with HELZ. Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ. Interacts with HSP90AA1; this interaction enhances SMYD3 histone-lysine N-methyltransferase.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family.
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