TRIM14 Antibody - #DF12490
| Product: | TRIM14 Antibody |
| Catalog: | DF12490 |
| Description: | Rabbit polyclonal antibody to TRIM14 |
| Application: | WB IHC |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Rabbit |
| Mol.Wt.: | 50 kDa; 50kD(Calculated). |
| Uniprot: | Q14142 |
| RRID: | AB_2845295 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12490, RRID:AB_2845295.
Fold/Unfold
5830400N10Rik; KIAA0129; pub; TRI14_HUMAN; TRIM14; tripartite motif protein 14; tripartite motif protein TRIM14; tripartite motif-containing 14; Tripartite motif-containing protein 14;
Immunogens
- Q14142 TRI14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAATGSRTPGRSELVEGCGWRCPEHGDRVAELFCRRCRRCVCALCPVLGAHRGHPVGLALEAAVHVQKLSQECLKQLAIKKQQHIDNITQIEDATEKLKANAESSKTWLKGKFTELRLLLDEEEALAKKFIDKNTQLTLQVYREQADSCREQLDIMNDLSNRVWSISQEPDPVQRLQAYTATEQEMQQQMSLGELCHPVPLSFEPVKSFFKGLVEAVESTLQTPLDIRLKESINCQLSDPSSTKPGTLLKTSPSPERSLLLKYARTPTLDPDTMHARLRLSADRLTVRCGLLGSLGPVPVLRFDALWQVLARDCFATGRHYWEVDVQEAGAGWWVGAAYASLRRRGASAAARLGCNRQSWCLKRYDLEYWAFHDGQRSRLRPRDDLDRLGVFLDYEAGVLAFYDVTGGMSHLHTFRATFQEPLYPALRLWEGAISIPRLP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14142 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K365 | Ubiquitination | Uniprot |
Research Backgrounds
Plays a role in the innate immune defense against viruses. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation.
Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination; this modification allows IKBKG/NEMO recruitment to MAVS.
Mitochondrion outer membrane.
Highest expression in liver; undetectable in skeletal muscle.
Interacts with MAVS. Interacts with WRNIP1 and PPP6C; these interactions positively regulate the RIG-I/DDX58 signaling pathway. Interacts with CGAS; this interaction stabilizes CGAS and promotes type I interferon production. Interacts with USP14; this interaction mediates the cleavage of 'Lys-48'-linked ubiquitination of CGAS. Interacts with SPI1 (By similarity).
The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.
Belongs to the TRIM/RBCC family.
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