USP9X Antibody - #DF12498

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Product: USP9X Antibody
Catalog: DF12498
Description: Rabbit polyclonal antibody to USP9X
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 260-290 kDa; 292kD(Calculated).
Uniprot: Q93008
RRID: AB_2845303

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
USP9X Antibody detects endogenous levels of total USP9X.
RRID:
AB_2845303
Cite Format: Affinity Biosciences Cat# DF12498, RRID:AB_2845303.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deubiquitinating enzyme FAF X; Deubiquitinating enzyme FAF-X; DFFRX; Drosophila fat facets related X linked; FAF; Fafl; Fam; Fat facets homolog; Fat facets in mammals; Fat facets protein related X linked; Fat facets protein related, X-linked; Fat facets protein-related; hFAM; MRX99; Probable ubiquitin carboxyl terminal hydrolase FAF X; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; Ubiquitin carboxyl-terminal hydrolase FAM; Ubiquitin specific peptidase 9 X linked; Ubiquitin specific peptidase 9, X-linked; Ubiquitin specific processing protease FAF X; Ubiquitin specific protease 9 X chromosome; Ubiquitin thioesterase FAF X; Ubiquitin thiolesterase FAF X; Ubiquitin thiolesterase FAF-X; Ubiquitin-specific protease 9; Ubiquitin-specific-processing protease FAF-X; USP9 (gene name); Usp9x; USP9X_HUMAN; Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila); X chromosome; X-linked;

Immunogens

Immunogen:
Uniprot:

Q93008(USP9X_HUMAN) >>Visit HPA database.

Gene(ID):
USP9X(8239)
Expression:
Q93008 USP9X_HUMAN:

Widely expressed in embryonic and adult tissues.

Sequence:
MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQLEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGNPEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLYARDHEDYDPQTVRLGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSVNCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFVVRFPNQGRQVDDLEVWSHTNDTIGSVRRCILNRIKANVAHTKIELFVGGELIDPADDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMPPDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTQINQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWASGCGSLQLVFSPNEEITKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGIEETILEGHLGVTKELLAFQTSEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPPTINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDKPALSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISELAITTRPHQIIMPSAIERSVRKQNVQFMHNRMQYSMEYFQFMKKLLTCNGVYLNPPPGQDHLLPEAEEITMISIQLAARFLFTTGFHTKKVVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSSINGNPPLPNPFGDPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEVKKATSVQQIEMEESKEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
92
Sheep
92
Pig
56
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q93008 As Substrate

Site PTM Type Enzyme
K112 Ubiquitination
S199 Phosphorylation
K218 Ubiquitination
K303 Acetylation
K303 Ubiquitination
S308 Phosphorylation
S313 Phosphorylation
K315 Acetylation
K315 Ubiquitination
K329 Ubiquitination
Y367 Phosphorylation
Y368 Phosphorylation
S403 Phosphorylation
Y409 Phosphorylation
K424 Ubiquitination
K441 Ubiquitination
K447 Ubiquitination
K486 Ubiquitination
K548 Ubiquitination
R560 Methylation
K571 Ubiquitination
S588 Phosphorylation
T590 Phosphorylation
S593 Phosphorylation
K666 Ubiquitination
K678 Ubiquitination
Y701 Phosphorylation
S702 Phosphorylation
K745 Ubiquitination
K794 Ubiquitination
Y797 Phosphorylation
R844 Methylation
S866 Phosphorylation
Y868 Phosphorylation
S887 Phosphorylation
T930 Phosphorylation
K958 Ubiquitination
T984 Phosphorylation
K1045 Ubiquitination
K1066 Ubiquitination
Y1148 Phosphorylation
K1153 Ubiquitination
Y1232 Phosphorylation
K1370 Ubiquitination
Y1375 Phosphorylation
K1435 Ubiquitination
K1445 Ubiquitination
K1446 Ubiquitination
K1453 Ubiquitination
S1580 Phosphorylation
T1591 Phosphorylation
S1593 Phosphorylation
S1600 Phosphorylation
S1609 Phosphorylation
Y1620 Phosphorylation
K1627 Ubiquitination
K1632 Ubiquitination
K1637 Ubiquitination
K1669 Ubiquitination
K1712 Ubiquitination
K1722 Acetylation
K1722 Ubiquitination
K1773 Ubiquitination
K1798 Ubiquitination
K1811 Ubiquitination
Y1815 Phosphorylation
K1862 Ubiquitination
K1899 Ubiquitination
K1935 Ubiquitination
K2219 Ubiquitination
K2301 Ubiquitination
K2360 Ubiquitination
R2366 Methylation
T2438 Phosphorylation
Y2439 Phosphorylation
S2443 Phosphorylation
S2448 Phosphorylation
Y2556 Phosphorylation
S2559 Phosphorylation
S2563 Phosphorylation
T2567 Phosphorylation

Research Backgrounds

Function:

Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration. Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein ARNTL/BMAL1 via its deubiquitinating activity.

Subcellular Location:

Cytoplasm. Cell projection>Growth cone.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in embryonic and adult tissues.

Subunit Structure:

Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin. Interacts with DCX. Interacts with OTUD4 and USP7; the interaction is direct. Interacts with ARNTL/BMAL1.

Family&Domains:

Belongs to the peptidase C19 family.

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