WDR1 Antibody - #DF12501

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Product: WDR1 Antibody
Catalog: DF12501
Description: Rabbit polyclonal antibody to WDR1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 66-70 kDa; 66kD(Calculated).
Uniprot: O75083
RRID: AB_2845306

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
WDR1 Antibody detects endogenous levels of total WDR1.
RRID:
AB_2845306
Cite Format: Affinity Biosciences Cat# DF12501, RRID:AB_2845306.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin interacting protein 1; Actin-interacting protein 1; AIP1; D5Wsu185e; NORI 1; NORI-1; WD repeat domain 1; WD repeat-containing protein 1; wdr1; WDR1_HUMAN;

Immunogens

Immunogen:
Uniprot:

O75083(WDR1_HUMAN) >>Visit HPA database.

Gene(ID):
WDR1(9948)
Sequence:
MPYEIKKVFASLPQVERGVSKIIGGDPKGNNFLYTNGKCVILRNIDNPALADIYTEHAHQVVVAKYAPSGFYIASGDVSGKLRIWDTTQKEHLLKYEYQPFAGKIKDIAWTEDSKRIAVVGEGREKFGAVFLWDSGSSVGEITGHNKVINSVDIKQSRPYRLATGSDDNCAAFFEGPPFKFKFTIGDHSRFVNCVRFSPDGNRFATASADGQIYIYDGKTGEKVCALGGSKAHDGGIYAISWSPDSTHLLSASGDKTSKIWDVSVNSVVSTFPMGSTVLDQQLGCLWQKDHLLSVSLSGYINYLDRNNPSKPLHVIKGHSKSIQCLTVHKNGGKSYIYSGSHDGHINYWDSETGENDSFAGKGHTNQVSRMTVDESGQLISCSMDDTVRYTSLMLRDYSGQGVVKLDVQPKCVAVGPGGYAVVVCIGQIVLLKDQRKCFSIDNPGYEPEVVAVHPGGDTVAIGGVDGNVRLYSILGTTLKDEGKLLEAKGPVTDVAYSHDGAFLAVCDASKVVTVFSVADGYSENNVFYGHHAKIVCLAWSPDNEHFASGGMDMMVYVWTLSDPETRVKIQDAHRLHHVSSLAWLDEHTLVTTSHDASVKEWTITY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Chicken
92
Horse
58
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75083 As Substrate

Site PTM Type Enzyme
Y3 Phosphorylation
K7 Ubiquitination
S11 Phosphorylation
R17 Methylation
S20 Phosphorylation
K21 Ubiquitination
K28 Acetylation
K28 Ubiquitination
Y34 Phosphorylation
K38 Acetylation
K38 Ubiquitination
Y54 Phosphorylation
T55 Phosphorylation
K65 Ubiquitination
Y72 Phosphorylation
S75 Phosphorylation
S79 Phosphorylation
K81 Acetylation
K81 Ubiquitination
R83 Methylation
K90 Ubiquitination
K95 Acetylation
K95 Ubiquitination
Y96 Phosphorylation
Y98 Phosphorylation
K104 Acetylation
K104 Ubiquitination
K106 Ubiquitination
K115 Acetylation
K115 Ubiquitination
K126 Ubiquitination
S151 Phosphorylation
K155 Ubiquitination
T164 Phosphorylation
S166 Phosphorylation
K180 Acetylation
K180 Ubiquitination
K182 Acetylation
K182 Ubiquitination
Y214 Phosphorylation
K219 Acetylation
K219 Ubiquitination
K223 Acetylation
K223 Ubiquitination
K231 Ubiquitination
Y238 Phosphorylation
S241 Phosphorylation
K256 Acetylation
K256 Ubiquitination
Y300 Phosphorylation
S310 Phosphorylation
K311 Ubiquitination
K317 Ubiquitination
K321 Acetylation
K321 Ubiquitination
S322 Phosphorylation
T327 Phosphorylation
K330 Acetylation
K330 Ubiquitination
K334 Ubiquitination
Y348 Phosphorylation
S351 Phosphorylation
S358 Phosphorylation
K362 Ubiquitination
S383 Phosphorylation
S392 Phosphorylation
R396 Methylation
Y398 Phosphorylation
S399 Phosphorylation
K405 Ubiquitination
K437 Ubiquitination
Y472 Phosphorylation
S473 Phosphorylation
K480 Acetylation
K480 Ubiquitination
K484 Ubiquitination
K489 Ubiquitination
K569 Acetylation
K569 Ubiquitination
S580 Phosphorylation
S581 Phosphorylation

Research Backgrounds

Function:

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing (By similarity). Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance (By similarity). Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension (By similarity). Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Podosome. Cell junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the WD repeat AIP1 family.

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