WDR18 Antibody - #DF12502
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12502, RRID:AB_2845307.
Fold/Unfold
Involved in Processing ITS2 3 homolog; Ipi3; MGC2436; R32184_1; WD repeat domain 18; WD repeat-containing protein 18; Wdr18; WDR18_HUMAN;
Immunogens
- Q9BV38 WDR18_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPMEVAVCTDSAAPMWSCIVWELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQLGKNYISAWELQRKDQLQQKIMCPGPVTCLTASPNGLYVLAGVAESIHLWEVSTGNLLVILSRHYQDVSCLQFTGDSSHFISGGKDCLVLVWSLCSVLQADPSRIPAPRHVWSHHALPITDLHCGFGGPLARVATSSLDQTVKLWEVSSGELLLSVLFDVSIMAVTMDLAEHHMFCGGSEGSIFQVDLFTWPGQRERSFHPEQDAGKVFKGHRNQVTCLSVSTDGSVLLSGSHDETVRLWDVQSKQCIRTVALKGPVTNAAILLAPVSMLSSDFRPSLPLPHFNKHLLGAEHGDEPRHGGLTLRLGLHQQGSEPSYLDRTEQLQAVLCSTMEKSVLGGQDQLRVRVTELEDEVRNLRKINRDLFDFSTRFITRPAK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BV38 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y51 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| Y372 | Phosphorylation | Uniprot | |
| R375 | Methylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot |
Research Backgrounds
Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit.
Nucleus>Nucleolus. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions.
Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9. Component of the PELP1 complex, composed of at least PELP1, TEX10 and WDR18. The complex interacts with pre-60S ribosome particles.
Belongs to the WD repeat IPI3/WDR18 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.