WSB1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/5

WSB1 Antibody - Western blot analysis of extracts from COLO 320 cells, using WSB1 antibody.

WSB1 Antibody - DF12505 at 1/100 staining Mouse liver tissue by IHC-P.

WSB1 Antibody - DF12505 at 1/100 staining Mouse testis tissue by IHC-P.

WSB1 Antibody - DF12505 staining Hela cells by IF/ICC.

$Figure 3. Subpopulation analysis of microglia clusters under homeostatic conditions. (A) Pseudotime plot (from monocle3) shows the differentiation trajectory from Mic_home to Mic_pre2 cells. (B) Line plot of the fraction of cells in the Mic_home, Mic_pre1, and Mic_pre2 subpopulations over time. (C) Expression of the ten genes with the highest Moran’s I score alongside the pseudotime trajectory. (D) Venn plot of the mutual and unique marker genes for the Mic_home, Mic_pre1, and Mic_pre2 subpopulations. (E) Volcano plot displays the differentially expressed genes between Mic_home and Mic_pre2 cells. Red dots represent the upregulated genes in the Mic_home cluster compared with the Mic_pre2 cluster and blue dots represent the downregulated genes. (F) Violin plot of the gene set variation analysis (GSVA) scores for important functional pathways of the Mic_home, Mic_pre1, and Mic_pre2 subpopulations. (G) Double immunofluorescence staining of Mic_home cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), DAPI (blue, representing cell nuclei), and Hpgd (red, representing the marker of Mic_home cells) antibodies (N=3). The yellow bar represents 100μm. (H) Double immunofluorescence staining of Mic_pre2 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), DAPI (blue, representing cell nuclei), and Wsb1 (red, representing the marker of Mic_pre2 cells) antibodies (N=3). The yellow bar represents 100μm. (I) Bar plot of the ratio of Hpgd+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6). (J) Bar plot of the ratio of Wsb1+Iba1+ cells (green plus red) compared with DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6).$

WSB1 Antibody - Figure 3.

Product:	WSB1 Antibody
Catalog:	DF12505
Description:	Rabbit polyclonal antibody to WSB1
Application:	WB IHC IF/ICC
Cited expt.:	IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	56 kDa; 47kD(Calculated).
Uniprot:	Q9Y6I7
RRID:	AB_2845310

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

WSB1 Antibody detects endogenous levels of total WSB1.

RRID:

AB_2845310
Cite Format: Affinity Biosciences Cat# DF12505, RRID:AB_2845310.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

SOCS box containing WD protein SWiP 1; SWIP1; WD repeat and SOCS box containing 1; WD repeat and SOCS box containing protein 1; WSB 1;

Immunogens

Immunogen:

A synthesized peptide derived from human WSB1, corresponding to a region within the internal amino acids.

Uniprot:

Q9Y6I7(WSB1_HUMAN) >>Visit HPA database.

Gene(ID):

WSB1(26118)

Sequence:

Q9Y6I7 WSB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASFPPRVNEKEIVRLRTIGELLAPAAPFDKKCGRENWTVAFAPDGSYFAWSQGHRTVKLVPWSQCLQNFLLHGTKNVTNSSSLRLPRQNSDGGQKNKPREHIIDCGDIVWSLAFGSSVPEKQSRCVNIEWHRFRFGQDQLLLATGLNNGRIKIWDVYTGKLLLNLVDHTEVVRDLTFAPDGSLILVSASRDKTLRVWDLKDDGNMMKVLRGHQNWVYSCAFSPDSSMLCSVGASKAVFLWNMDKYTMIRKLEGHHHDVVACDFSPDGALLATASYDTRVYIWDPHNGDILMEFGHLFPPPTPIFAGGANDRWVRSVSFSHDGLHVASLADDKMVRFWRIDEDYPVQVAPLSNGLCCAFSTDGSVLAAGTHDGSVYFWATPRQVPSLQHLCRMSIRRVMPTQEVQELPIPSKLLEFLSYRI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Zebrafish

100

Rabbit

100

Sheep

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9Y6I7_WSB1_HUMAN

Function:

Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes type II iodothyronine deiodinase/DIO2. Confers constitutive instability to HIPK2 through proteasomal degradation.

Family&Domains:

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.

References

1). Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke. Aging and disease, 2023 (PubMed: 37199734) [IF=7.0]

Application: IF/ICC Species: Mouse Sample:

Figure 3. Subpopulation analysis of microglia clusters under homeostatic conditions. (A) Pseudotime plot (from monocle3) shows the differentiation trajectory from Mic_home to Mic_pre2 cells. (B) Line plot of the fraction of cells in the Mic_home, Mic_pre1, and Mic_pre2 subpopulations over time. (C) Expression of the ten genes with the highest Moran’s I score alongside the pseudotime trajectory. (D) Venn plot of the mutual and unique marker genes for the Mic_home, Mic_pre1, and Mic_pre2 subpopulations. (E) Volcano plot displays the differentially expressed genes between Mic_home and Mic_pre2 cells. Red dots represent the upregulated genes in the Mic_home cluster compared with the Mic_pre2 cluster and blue dots represent the downregulated genes. (F) Violin plot of the gene set variation analysis (GSVA) scores for important functional pathways of the Mic_home, Mic_pre1, and Mic_pre2 subpopulations. (G) Double immunofluorescence staining of Mic_home cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), DAPI (blue, representing cell nuclei), and Hpgd (red, representing the marker of Mic_home cells) antibodies (N=3). The yellow bar represents 100μm. (H) Double immunofluorescence staining of Mic_pre2 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), DAPI (blue, representing cell nuclei), and Wsb1 (red, representing the marker of Mic_pre2 cells) antibodies (N=3). The yellow bar represents 100μm. (I) Bar plot of the ratio of Hpgd+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6). (J) Bar plot of the ratio of Wsb1+Iba1+ cells (green plus red) compared with DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6).

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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WSB1 Antibody - #DF12505