Phospho-CD79A (Tyr182) Antibody - #AF2329

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Product: Phospho-CD79A (Tyr182) Antibody
Catalog: AF2329
Description: Rabbit polyclonal antibody to Phospho-CD79A (Tyr182)
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 45-55kDa; 25kD(Calculated).
Uniprot: P11912
RRID: AB_2845343

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Phospho-CD79A (Tyr182) Antibody detects endogenous levels of CD79A only when phosphorylated at Tyr182.
RRID:
AB_2845343
Cite Format: Affinity Biosciences Cat# AF2329, RRID:AB_2845343.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B lymphocyte-specific MB1 protein; B-cell antigen receptor complex-associated protein alpha chain; CD 79a; CD79a; CD79a antigen (immunoglobulin-associated alpha); CD79A antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A_HUMAN; Ig alpha; Ig-alpha; IGA; IgM-alpha; Immunoglobulin-associated alpha; Ly54; MB-1 membrane glycoprotein; MB1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein;

Immunogens

Immunogen:

A synthesized peptide derived from human CD79A around the phosphorylation site of Tyr182.

Uniprot:

P11912(CD79A_HUMAN) >>Visit HPA database.

Gene(ID):
CD79A(973)
Expression:
P11912 CD79A_HUMAN:

B-cells.

Sequence:
MPGGPGVLQALPATIFLLFLLSAVYLGPGCQALWMHKVPASLMVSLGEDAHFQCPHNSSNNANVTWWRVLHGNYTWPPEFLGPGEDPNGTLIIQNVNKSHGGIYVCRVQEGNESYQQSCGTYLRVRQPPPRPFLDMGEGTKNRIITAEGIILLFCAVVPGTLLLFRKRWQNEKLGLDAGDEYEDENLYEGLNLDDCSMYEDISRGLQGTYQDVGSLNIGDVQLEKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
83
Xenopus
80
Pig
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

PTMs:

Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

B-cells.

Research Fields

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

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