Product: Phospho-TBC1D1 (Thr590) Antibody
Catalog: AF2422
Description: Rabbit polyclonal antibody to Phospho-TBC1D1 (Thr590)
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 160kDa;
Uniprot: Q60949
RRID: AB_2819092

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-TBC1D1 (Thr590) Antibody detects endogenous levels of TBC1D1 only when phosphorylated at Thr590.
RRID:
AB_2819092
Cite Format: Affinity Biosciences Cat# AF2422, RRID:AB_2819092.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA1108; TBC 1; TBC; TBC1 (tre 2/USP6, BUB2, cdc16) domain family, member 1; TBC1; TBC1 domain family member 1; Tbc1d1; TBCD1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from mouse TBC1D1 around the phosphorylation site of Thr590.

References

1). HWL-088, a new highly potent FFAR1 agonist, improves glucolipid metabolism and acts additively with Metformin in ob/ob diabetic mice. BRITISH JOURNAL OF PHARMACOLOGY, 2020 (PubMed: 31971610) [IF=6.8]

Application: WB    Species: mice    Sample: liver

FIGURE 4 Effects of HWL-088 on plasma glucose levels (a) and corresponding AUC0–90min (b) of glucose during an OGTT in fasting ob/ob mice. Tested agents or vehicle was orally administered to ob/ob mice twice daily for 30 days, and the OGTTs were determined on treatment days 1, 15, and 30. After long-term treatment, hepatic expression of genes involved in gluconeogenesis (c) and glycogen synthesis (d) as well as hepatic expression of GSK3β (e). Effects of HWL-088 on the phosphorylation of AKT, AS160, and TBC1D1 in L6 myoblasts cells (f). Values are mean ± SD (n = 6). *P ≤ .05 was analysed using a one-way ANOVA with Tukey's multiple-comparison post hoc test

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