Phospho-TBC1D1 (Thr590) Antibody - #AF2422
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| Product: | Phospho-TBC1D1 (Thr590) Antibody |
| Catalog: | AF2422 |
| Description: | Rabbit polyclonal antibody to Phospho-TBC1D1 (Thr590) |
| Application: | WB IHC |
| Cited expt.: | WB |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 160kDa; |
| Uniprot: | Q60949 |
| RRID: | AB_2819092 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-TBC1D1 (Thr590) Antibody detects endogenous levels of TBC1D1 only when phosphorylated at Thr590.
RRID:
AB_2819092
Cite Format: Affinity Biosciences Cat# AF2422, RRID:AB_2819092.
Cite Format: Affinity Biosciences Cat# AF2422, RRID:AB_2819092.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
KIAA1108; TBC 1; TBC; TBC1 (tre 2/USP6, BUB2, cdc16) domain family, member 1; TBC1; TBC1 domain family member 1; Tbc1d1; TBCD1_HUMAN;
Immunogens
Immunogen:
A synthesized peptide derived from mouse TBC1D1 around the phosphorylation site of Thr590.
References
1). HWL-088, a new highly potent FFAR1 agonist, improves glucolipid metabolism and acts additively with Metformin in ob/ob diabetic mice. BRITISH JOURNAL OF PHARMACOLOGY, 2020
(PubMed: 31971610)
[IF=6.8]
Application: WB Species: mice Sample: liver
FIGURE 4 Effects of HWL-088 on plasma glucose levels (a) and corresponding AUC0–90min (b) of glucose during an OGTT in fasting ob/ob
mice. Tested agents or vehicle was orally administered to ob/ob mice twice daily for 30 days, and the OGTTs were determined on treatment days
1, 15, and 30. After long-term treatment, hepatic expression of genes involved in gluconeogenesis (c) and glycogen synthesis (d) as well as hepatic
expression of GSK3β (e). Effects of HWL-088 on the phosphorylation of AKT, AS160, and TBC1D1 in L6 myoblasts cells (f). Values are
mean ± SD (n = 6). *P ≤ .05 was analysed using a one-way ANOVA with Tukey's multiple-comparison post hoc test
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