SMC4 Antibody - #DF12518

(2)  
Product: SMC4 Antibody
Catalog: DF12518
Description: Rabbit polyclonal antibody to SMC4
Application: WB
Reactivity: Human, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 150 kDa; 147kD(Calculated).
Uniprot: Q9NTJ3
RRID: AB_2845480

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(90%), Horse(90%), Sheep(90%), Rabbit(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
SMC4 Antibody detects endogenous levels of total SMC4.
RRID:
AB_2845480
Cite Format: Affinity Biosciences Cat# DF12518, RRID:AB_2845480.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAP C; CAPC; Chromosome associated polypeptide C; Chromosome-associated polypeptide C; hCAP C; hCAP-C; SMC 4; SMC protein 4; SMC-4; SMC4; SMC4_HUMAN; SMC4L1; Structural maintenance of chromosomes 4; Structural maintenance of chromosomes 4 like 1; Structural maintenance of chromosomes protein 4; XCAP C homolog; XCAP-C homolog;

Immunogens

Immunogen:
Uniprot:

Q9NTJ3(SMC4_HUMAN) >>Visit HPA database.

Gene(ID):
SMC4(10051)
Expression:
Q9NTJ3 SMC4_HUMAN:

Widely expressed. Higher expression in testis, colon, thymus.

Sequence:
MPRKGTQPSTARRREEGPPPPSPDGASSDAEPEPPSGRTESPATAAETASEELDNRSLEEILNSIPPPPPPAMTNEAGAPRLMITHIVNQNFKSYAGEKILGPFHKRFSCIIGPNGSGKSNVIDSMLFVFGYRAQKIRSKKLSVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNIINETTTRNNALEKEKEKEEKKLKEVMDSLKQETQGLQKEKESREKELMGFSKSVNEARSKMDVAQSELDIYLSRHNTAVSQLTKAKEALIAASETLKERKAAIRDIEGKLPQTEQELKEKEKELQKLTQEETNFKSLVHDLFQKVEEAKSSLAMNRSRGKVLDAIIQEKKSGRIPGIYGRLGDLGAIDEKYDVAISSCCHALDYIVVDSIDIAQECVNFLKRQNIGVATFIGLDKMAVWAKKMTEIQTPENTPRLFDLVKVKDEKIRQAFYFALRDTLVADNLDQATRVAYQKDRRWRVVTLQGQIIEQSGTMTGGGSKVMKGRMGSSLVIEISEEEVNKMESQLQNDSKKAMQIQEQKVQLEERVVKLRHSEREMRNTLEKFTASIQRLIEQEEYLNVQVKELEANVLATAPDKKKQKLLEENVSAFKTEYDAVAEKAGKVEAEVKRLHNTIVEINNHKLKAQQDKLDKINKQLDECASAITKAQVAIKTADRNLQKAQDSVLRTEKEIKDTEKEVDDLTAELKSLEDKAAEVVKNTNAAEESLPEIQKEHRNLLQELKVIQENEHALQKDALSIKLKLEQIDGHIAEHNSKIKYWHKEISKISLHPIEDNPIEEISVLSPEDLEAIKNPDSITNQIALLEARCHEMKPNLGAIAEYKKKEELYLQRVAELDKITYERDSFRQAYEDLRKQRLNEFMAGFYIITNKLKENYQMLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSWKKIFNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISDRLIGIYKTYNITKSVAVNPKEIASKGLC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Sheep
90
Dog
80
Rabbit
80
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NTJ3 As Substrate

Site PTM Type Enzyme
Phosphorylation
K4 Methylation
S22 Phosphorylation
S27 Phosphorylation
S28 Phosphorylation
S36 Phosphorylation
T39 Phosphorylation
S41 Phosphorylation
T44 Phosphorylation
T48 Phosphorylation
S50 Phosphorylation
T85 Phosphorylation
K93 Methylation
K93 Ubiquitination
K99 Ubiquitination
K106 Ubiquitination
S109 Phosphorylation
K119 Methylation
K141 Ubiquitination
S143 Phosphorylation
S149 Phosphorylation
K153 Ubiquitination
K170 Ubiquitination
Y175 Phosphorylation
T188 Phosphorylation
Y197 Phosphorylation
S200 Phosphorylation
K202 Ubiquitination
K203 Ubiquitination
K207 Ubiquitination
R214 Methylation
K241 Ubiquitination
K268 Acetylation
K268 Ubiquitination
R281 Methylation
K293 Ubiquitination
K332 Ubiquitination
K341 Ubiquitination
K343 Ubiquitination
T348 Phosphorylation
K349 Acetylation
K349 Ubiquitination
K354 Acetylation
K354 Ubiquitination
S355 Phosphorylation
K363 Acetylation
K363 Ubiquitination
K381 Acetylation
K381 Ubiquitination
K389 Ubiquitination
K413 Ubiquitination
K416 Acetylation
K416 Ubiquitination
K434 Ubiquitination
S489 Phosphorylation
K522 Ubiquitination
S531 Phosphorylation
T533 Phosphorylation
K547 Ubiquitination
K564 Ubiquitination
K573 Acetylation
K573 Ubiquitination
S574 Phosphorylation
K582 Ubiquitination
K587 Ubiquitination
S588 Phosphorylation
S589 Phosphorylation
S595 Phosphorylation
K598 Acetylation
K598 Ubiquitination
K607 Acetylation
K607 Ubiquitination
K608 Acetylation
Y616 Phosphorylation
K679 Acetylation
T682 Phosphorylation
T686 Phosphorylation
K698 Ubiquitination
Y709 Phosphorylation
T739 Phosphorylation
K789 Ubiquitination
K797 Ubiquitination
K820 Ubiquitination
T822 Phosphorylation
K857 Ubiquitination
K867 Ubiquitination
K876 Ubiquitination
K898 Ubiquitination
K922 Ubiquitination
K928 Ubiquitination
K936 Ubiquitination
K953 Ubiquitination
K963 Ubiquitination
K968 Ubiquitination
K974 Ubiquitination
S982 Phosphorylation
K988 Ubiquitination
K998 Ubiquitination
K1009 Acetylation
K1009 Ubiquitination
S1013 Phosphorylation
K1015 Ubiquitination
S1030 Phosphorylation
K1031 Acetylation
K1031 Ubiquitination
K1037 Acetylation
K1037 Ubiquitination
K1041 Ubiquitination
S1043 Phosphorylation
S1056 Phosphorylation
S1059 Phosphorylation
K1067 Ubiquitination
K1087 Ubiquitination
K1097 Ubiquitination
K1098 Ubiquitination
K1099 Ubiquitination
K1112 Ubiquitination
Y1115 Phosphorylation
K1145 Ubiquitination
K1187 Ubiquitination
Y1266 Phosphorylation
K1267 Ubiquitination
K1273 Ubiquitination
K1280 Ubiquitination
K1285 Ubiquitination
C1288 S-Nitrosylation

Research Backgrounds

Function:

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Higher expression in testis, colon, thymus.

Subunit Structure:

Forms a heterodimer with SMC2. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG.

Family&Domains:

The SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.

Belongs to the SMC family. SMC4 subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.