Product: PDLIM3 Antibody
Catalog: DF12525
Description: Rabbit polyclonal antibody to PDLIM3
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Dog
Mol.Wt.: 35,39 kDa; 39kD(Calculated).
Uniprot: Q53GG5
RRID: AB_2845487

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(83%)
Clonality:
Polyclonal
Specificity:
ALP Antibody detects endogenous levels of total ALP.
RRID:
AB_2845487
Cite Format: Affinity Biosciences Cat# DF12525, RRID:AB_2845487.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actinin associated LIM protein; Actinin-associated LIM protein; Alpha actinin 2 associated LIM protein; Alpha-actinin-2-associated LIM protein; DKFZp686L0362; Enigma homolog; PDLI3_HUMAN; PDLIM 3; PDLIM3; PDZ and LIM domain 3; PDZ and LIM domain protein 3; ALP;PDLI3;

Immunogens

Immunogen:

A synthesized peptide derived from human ALP, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q53GG5 PDLI3_HUMAN:

Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.

Sequence:
MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQDGNYFEHKHNIRPKPFVIPGRSSGCSTPSGIDCGSGRSTPSSVSTVSTICPGDLKVAAKLAPNIPLEMELPGVKIVHAQFNTPMQLYSDDNIMETLQGQVSTALGETPLMSEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Pig
71
Horse
67
Bovine
67
Sheep
67
Chicken
57
Rabbit
57
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May play a role in the organization of actin filament arrays within muscle cells.

Subcellular Location:

Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Localizes to myofiber Z-lines.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.

References

1). Osteoblastic and anti-osteoclastic activities of strontium-substituted silicocarnotite ceramics: In vitro and in vivo studies. Bioactive Materials, 2020 (PubMed: 32280833) [IF=18.9]

Application: IHC    Species: Mice    Sample: bone and osteoid tissues

Fig. 9 (A) Masson staining: the region between surrounding bone and scaffold (40x & 100x); (B) TRAP staining: TRAP positive cells (black arrows); (C) IHC staining of RUNX-2; (D) IHC staining of ALP; (E) Quantitative analysis for TRAP staining and IHC staining. * Compared with CPS group; # compared with Sr-5 group.

2). Fusion peptide engineered “statically-versatile” titanium implant simultaneously enhancing anti-infection, vascularization and osseointegration. Biomaterials, 2021 (PubMed: 33069134) [IF=12.8]

3). On-demand storage and release of antimicrobial peptides using Pandora's box-like nanotubes gated with a bacterial infection-responsive polymer. Theranostics, 2020 (PubMed: 31903109) [IF=12.4]

Application: WB    Species: Human    Sample: Human bone mesenchymal stem cells(hBMSCs)

Figure 5. In vitro biocompatibilities and osteogenic activities of the substrates. (A) CCK-8 assay of hBMSCs on the indicated surfaces after 1, 3 and 7 days of culture. * denotes p < 0.05 and ** denotes p < 0.01 compared to Ti-NTs, # denotes p < 0.05 compared to the corresponding control group without peptides. Error bars denote the standard deviations over quadruplicate measurements with separately implants. (B) Confocal fluorescence microscopy images of hBMSCs stained with vinculin, F-actin and DAPI after being cultured for 24 h. Scale bar, 50 μm. (C-F) qRT-PCR assay of osteogenic gene expression of (C) ALP, (D) RUNX-2, (E) COL1 and (F) OPN of hBMSCs after 7 and 14 days of culture. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 compared to Ti-NTs-P-A; # denotes p < 0.05, & denotes p < 0.01 and $ denotes p < 0.001 compared to the corresponding control group without peptides. All error bars denote the standard deviations over quadruplicate measurements with separately implants. (G) Immunofluorescence staining of hBMSCs cultured on Ti-NTs-P-A for 7 days (ALP and RUNX-2) and 14 days (OPN). The images were obtained by confocal fluorescence microscopy. Scale bar, 50 μm. (H) Western blotting of hBMSCs cultured on the substrates for 7 and 14 days. At each time point, left lane was Ti-NTs, middle lane was Ti-NTs-A and right lane was Ti-NTs-P-A. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 compared to Ti-NTs-P-A. Error bars denote the standard deviations over triplicate measurements with separately Western blotting results.

4). ZIF-8 Modified Multifunctional Bone-Adhesive Hydrogels Promoting Angiogenesis and Osteogenesis for Bone Regeneration. ACS Applied Materials & Interfaces, 2020 (PubMed: 32814397) [IF=8.3]

5). MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway. Stem Cell Research & Therapy, 2019 (PubMed: 30704530) [IF=7.5]

Application: WB    Species: rat    Sample: BMMSCs

Fig. 4 |Inactivation of ERK/MAPK signaling pathway inhibited the odontogenic differentiation of BMMSCs induced by TGC-CM. a ERK inhibitor U0126 significantly inhibited the mineralization and b reduced ALP, DSP, and DMP-1 protein levels of BMMSCs during odontogenic differentiation induced by TGC-CM for 7 days. Scale bar = 100 μm

6). Insulin promotes the bone formation capability of human dental pulp stem cells through attenuating the IIS/PI3K/AKT/mTOR pathway axis. Stem cell research & therapy, 2024 (PubMed: 39075596) [IF=7.5]

Application: WB    Species: Human    Sample: DPSCs

Fig. 3 10− 6 M insulin promotes the osteogenic differentiation of human DPSCs. A 10− 6 M insulin up-regulated the mRNA levels of COL-1, ALP, OCN, and RUNX2 in DPSCs at day 3 and day 7 (n = 3). B 10− 6 M insulin promoted the protein expressions of COL-1, ALP, OCN, and RUNX2 in DPSCs at day 7 (n = 3). Representative western blotting (left) and quantification analysis (right). Full-length blots/gels are presented in Supplementary Fig. 1. C 10− 6 M insulin increased the secretion of extracellular bone metabolism and biochemical markers in DPSCs at day 1–7 and day 7–14 (n = 6). D 10− 6 M insulin enhanced alkaline phosphatase staining of DPSCs at day 21 (scale bars: 100 μm). E 10− 6 M insulin enhanced Alizarin red staining of DPSCs at day 21 (scale bars: 100 μm). F 10− 6 M insulin promoted the mineralized matrix formation of DPSCs at day 21 (n = 6). Data are expressed as the mean ± SD. *P 

7). miR-155-5p/Bmal1 Modulates the Senescence and Osteogenic Differentiation of Mouse BMSCs through the Hippo Signaling Pathway. Stem cell reviews and reports, 2024 (PubMed: 38150082) [IF=4.5]

8). Activation of osteoblast ferroptosis via the METTL3/ASK1‐p38 signaling pathway in high glucose and high fat (HGHF)‐induced diabetic bone loss. FASEB JOURNAL, 2022 (PubMed: 35104016) [IF=4.4]

9). Easily attainable and low immunogenic stem cells from exfoliated deciduous teeth enhanced the in vivo bone regeneration ability of gelatin/bioactive glass microsphere composite scaffolds. Frontiers in bioengineering and biotechnology, 2022 (PubMed: 36568292) [IF=4.3]

Application: IHC    Species: human    Sample:

FIGURE 6. Immunohistochemical staining of ALP, COL1, OCN, and RUNX2 after implantation for 4 and 8 weeks.

10). VX-765 ameliorates CKD VSMC calcification by regulating STAT3 activation. European Journal of Pharmacology, 2023 (PubMed: 36858340) [IF=4.2]

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