Product: TM158 Antibody
Catalog: DF12531
Description: Rabbit polyclonal antibody to TM158
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Chicken
Mol.Wt.: 30 kDa; 30kD(Calculated).
Uniprot: Q8WZ71
RRID: AB_2845493

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
TM158 Antibody detects endogenous levels of total TM158.
RRID:
AB_2845493
Cite Format: Affinity Biosciences Cat# DF12531, RRID:AB_2845493.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40 kDa BINP binding protein; 40 kDa BINP-binding protein; BBP; BINP receptor; Brain injury derived neurotrophic peptide (BINP) binding protein; Brain specific binding protein; DKFZp586E1621; HBBP; p40BBP; Ras induced senescence protein 1; Ras-induced senescence protein 1; RIS1; TM158_HUMAN; TMEM 158; TMEM158; Transmembrane protein 158;

Immunogens

Immunogen:

A synthesized peptide derived from human TM158, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Sequence:
MLPLLAALLAAACPLPPVRGGAADAPGLLGVPSNASVNASSADEPIAPRLLASAAPGPPERPGPEEAAAAAAPCNISVQRQMLSSLLVRWGRPRGFQCDLLLFSTNAHGRAFFAAAFHRVGPPLLIEHLGLAAGGAQQDLRLCVGCGWVRGRRTGRLRPAAAPSAAAATAGAPTALPAYPAAEPPGPLWLQGEPLHFCCLDFSLEELQGEPGWRLNRKPIESTLVACFMTLVIVVWSVAALIWPVPIIAGFLPNGMEQRRTTASTTAATPAAVPAGTTAAAAAAAAAAAAAAVTSGVATK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Chicken
90
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide.

PTMs:

N-glycosylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TMEM158 family.

References

1). TMEM158 functions as an oncogene and promotes lung adenocarcinoma progression through the PI3K/AKT pathway via interaction with TWIST1. Experimental cell research, 2024 (PubMed: 38508329) [IF=3.3]

Application: WB    Species: human    Sample:

Fig. 1. Expression patterns of TMEM158 in LUAD patients. (A, B) Volcano plots depicting the DEGs in LUAD were generated utilizing both TCGA-LUAD and GSE140797 datasets. (C) The Venn diagram illustrated the overlapping DEGs between the TCGA-LUAD and GSE140797 datasets. (D, E) In the TCGA-LUAD dataset, TMEM158 expression revealed a significant increase in LUAD tissues in comparison with healthy tissues. (F) The analysis of the GSE140797 dataset has shown that TMEM158 expression is raised in LUAD tissues contrasted to normal tissues. (G) Expression of TMEM158 in different malignancy kinds was analyzed employing data acquired from the TCGA database. (H, I) Analysis of LUAD samples and nearby normal tissues was conducted utilizing western blotting and qRT–PCR. *p < 0.05; **p < 0.01; ***p < 0.001.

Application: IHC    Species: human    Sample: H1650 cells

Fig. 4. TMEM158 promotes migration and invasion while inhibiting cell apoptosis in LUAD cells. (A, B) The apoptotic activities of H1650 cells were assessed using flow cytometry. (C, D) The migratory capability of H1650 cells was assessed employing the wound healing assay. (E, F) The Transwell invasion assay was employed to assess the invasiveness of H1650 cells. *p < 0.05.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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