PRAME Antibody - #DF12547
| Product: | PRAME Antibody |
| Catalog: | DF12547 |
| Description: | Rabbit polyclonal antibody to PRAME |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Dog |
| Mol.Wt.: | 58 kDa; 58kD(Calculated). |
| Uniprot: | P78395 |
| RRID: | AB_2845509 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12547, RRID:AB_2845509.
Fold/Unfold
4930534P07Rik; Cancer/testis antigen 130; CT130; MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3;
Immunogens
- P78395 PRAME_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P78395 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K184 | Acetylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot |
Research Backgrounds
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
Nucleus. Cell membrane.
Expressed in testis. Detected in samples of kidney, brain and skin.
Interacts with RARA (via the ligand-binding domain); the interaction is direct and ligand (retinoic acid)-dependent. Interacts with EZH2; required to repress RAR signaling.
Belongs to the PRAME family.
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