KDM2B Antibody - #DF12550

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Product: KDM2B Antibody
Catalog: DF12550
Description: Rabbit polyclonal antibody to KDM2B
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 165 kDa; 153kD(Calculated).
Uniprot: Q8NHM5
RRID: AB_2845512

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KDM2B Antibody detects endogenous levels of total KDM2B.
RRID:
AB_2845512
Cite Format: Affinity Biosciences Cat# DF12550, RRID:AB_2845512.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

[Histone-H3]-lysine-36 demethylase 1B; CXXC-type zinc finger protein 2; CXXC2; F box and leucine rich repeat protein 10; F box protein FBL10; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; Fbl10; FBXL10; JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein; JHDM1B; JmjC domain-containing histone demethylation protein 1B; Jumonji C domain containing histone demethylase 1B; Jumonji domain-containing EMSY-interactor methyltransferase motif protein; kdm2b; KDM2B_HUMAN; Lysine (K) specific demethylase 2B; Lysine-specific demethylase 2B; PCCX2; Protein containing CXXC domain 2; Protein JEMMA; Protein-containing CXXC domain 2;

Immunogens

Immunogen:
Uniprot:

Q8NHM5(KDM2B_HUMAN) >>Visit HPA database.

Gene(ID):
KDM2B(84678)
Sequence:
MAGPQMGGSAEDHPPRKRHAAEKQKKKTVIYTKCFEFESATQRPIDRQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREALRVPLIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPEAQRDKLYNVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNAIAEMKYPKVKKYCLMSVKGCFTDFHIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIELKQGYTFFIPSGWIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSHLTQEYQRESMLIDAPRKPSIDGFSSDSWLEMEEEACDQQPQEEEEKDEEGEGRDRAPKPPTDGSTSPTSTPSEDQEALGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHADDDPSLAITGVPVVTWPKKTPKNRAVGRPKGKLGPASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQCIAPVLPHTAVCLVCGEAGKEDTVEEEEGKFNLMLMECSICNEIIHPGCLKIKESEGVVNDELPNCWECPKCNHAGKTGKQKRGPGFKYASNLPGSLLKEQKMNRDNKEGQEPAKRRSECEEAPRRRSDEHSKKVPPDGLLRRKSDDVHLRKKRKYEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSSLSPWWRSSLTYFQQQLKPGKEDKLFRKKRRSWKNAEDRMALANKPLRRFKQEPEDELPEAPPKTRESDHSRSSSPTAGPSTEGAEGPEEKKKVKMRRKRRLPNKELSRELSKELNHEIQRTENSLANENQQPIKSEPESEGEEPKRPPGICERPHRFSKGLNGTPRELRHQLGPSLRSPPRVISRPPPSVSPPKCIQMERHVIRPPPISPPPDSLPLDDGAAHVMHREVWMAVFSYLSHQDLCVCMRVCRTWNRWCCDKRLWTRIDLNHCKSITPLMLSGIIRRQPVSLDLSWTNISKKQLSWLINRLPGLRDLVLSGCSWIAVSALCSSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQMDNRSKLRNIVELRLAGLDITDASLRLIIRHMPLLSKLHLSYCNHVTDQSINLLTAVGTTTRDSLTEINLSDCNKVTDQCLSFFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEKLLQKLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NHM5 As Substrate

Site PTM Type Enzyme
Y50 Phosphorylation
S57 Phosphorylation
K73 Ubiquitination
Y94 Phosphorylation
K111 Ubiquitination
K117 Ubiquitination
T124 Phosphorylation
K129 Ubiquitination
S134 Phosphorylation
T145 Phosphorylation
K147 Ubiquitination
T149 Phosphorylation
K210 Ubiquitination
K222 Ubiquitination
Y223 Phosphorylation
Y229 Phosphorylation
T440 Phosphorylation
S443 Phosphorylation
T444 Phosphorylation
S445 Phosphorylation
T447 Phosphorylation
T449 Phosphorylation
T472 Phosphorylation
S474 Phosphorylation
S477 Phosphorylation
S480 Phosphorylation
T493 Phosphorylation
T495 Phosphorylation
S497 Phosphorylation
T500 Phosphorylation
S503 Phosphorylation
K515 Ubiquitination
K532 Ubiquitination
S593 Phosphorylation
Y739 Phosphorylation
S741 Phosphorylation
S746 Phosphorylation
K758 Acetylation
K765 Acetylation
S768 Phosphorylation
S795 Phosphorylation
S813 Phosphorylation
S820 Phosphorylation
T823 Phosphorylation
S824 Phosphorylation
S827 Phosphorylation
S832 Phosphorylation
S840 Phosphorylation
S842 Phosphorylation
T850 Phosphorylation
K884 Ubiquitination
K890 Methylation
K890 Sumoylation
S912 Phosphorylation
S913 Phosphorylation
S914 Phosphorylation
T916 Phosphorylation
S947 Phosphorylation
S951 Phosphorylation
S975 Phosphorylation
S979 Phosphorylation
S1015 Phosphorylation
S1018 Phosphorylation
S1029 Phosphorylation
S1031 Phosphorylation
K1111 Ubiquitination
K1187 Ubiquitination
R1231 Methylation

Research Backgrounds

Function:

Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (Probable).

Subcellular Location:

Nucleus>Nucleolus. Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SKP1, forming heterodimers. The heterodimeric KDM2B-SKP1 complex interacts with the PCGF1-BCORL1 heterodimeric complex to form a homotetrameric polycomb repression complex 1 (PRC1.1). Directly interacts with CUL1. The SKP1-KDM2B complex interacts with UBB.

Family&Domains:

The LRR repeats are required for the interaction with the PCGF1-BCORL1 heterodimeric complex.

The JmjC domain mediates demethylation activity (PubMed:17994099). It is also required for repression of ribosomal RNA genes (PubMed:17994099).

The CXXC zinc finger mediates binding to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides.

The F-box domain mediates interaction with UBB.

Belongs to the JHDM1 histone demethylase family.

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