USP5 Antibody | Affinity Biosciences

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USP5 Antibody - Western blot analysis of extracts from A549 cells, using UBP5 Antibody.

Product:	USP5 Antibody
Catalog:	DF12553
Description:	Rabbit polyclonal antibody to USP5
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Chicken
Mol.Wt.:	96 kDa; 96kD(Calculated).
Uniprot:	P45974
RRID:	AB_2845515

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

USP5 Antibody detects endogenous levels of total USP5.

RRID:

AB_2845515
Cite Format: Affinity Biosciences Cat# DF12553, RRID:AB_2845515.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deubiquitinating enzyme 5; Isopeptidase T; ISOT 1; ISOT; Ubiquitin carboxyl-terminal hydrolase 5; Ubiquitin isopeptidase T; Ubiquitin specific peptidase 5 (isopeptidase T); Ubiquitin specific peptidase 5; Ubiquitin specific processing protease 5; Ubiquitin specific protease 5 (isopeptidase T); Ubiquitin specific protease 5 (ubiquitin isopeptidase T); Ubiquitin thioesterase 5; Ubiquitin thiolesterase 5; Ubiquitin-specific-processing protease 5; UBP5_HUMAN; Usp5;

Immunogens

Immunogen:

Uniprot:

P45974(UBP5_HUMAN) >>Visit HPA database.

Gene(ID):

USP5(8078)

Sequence:

P45974 UBP5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Chicken

Xenopus

Zebrafish

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P45974 As Substrate

P45974

Site	PTM Type	Source
A2	Acetylation	Uniprot
S5	Phosphorylation	Uniprot
K20	Ubiquitination	Uniprot
K27	Ubiquitination	Uniprot
Y57	Phosphorylation	Uniprot
K64	Ubiquitination	Uniprot
T65	Phosphorylation	Uniprot
K80	Ubiquitination	Uniprot
Y128	Phosphorylation	Uniprot
S156	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
K163	Acetylation	Uniprot
K163	Ubiquitination	Uniprot
K178	Acetylation	Uniprot
K178	Ubiquitination	Uniprot
S182	Phosphorylation	Uniprot
K184	Acetylation	Uniprot
K184	Ubiquitination	Uniprot
K198	Ubiquitination	Uniprot
T213	Phosphorylation	Uniprot
S216	Phosphorylation	Uniprot
K247	Ubiquitination	Uniprot
T289	Phosphorylation	Uniprot
K291	Ubiquitination	Uniprot
T292	Phosphorylation	Uniprot
K318	Ubiquitination	Uniprot
K353	Acetylation	Uniprot
K353	Ubiquitination	Uniprot
K357	Acetylation	Uniprot
K357	Ubiquitination	Uniprot
K360	Sumoylation	Uniprot
K360	Ubiquitination	Uniprot
K379	Ubiquitination	Uniprot
Y389	Phosphorylation	Uniprot
K391	Ubiquitination	Uniprot
K406	Ubiquitination	Uniprot
K418	Ubiquitination	Uniprot
K423	Ubiquitination	Uniprot
S453	Phosphorylation	Uniprot
K468	Ubiquitination	Uniprot
K470	Ubiquitination	Uniprot
K476	Ubiquitination	Uniprot
Y479	Phosphorylation	Uniprot
Y485	Phosphorylation	Uniprot
K499	Ubiquitination	Uniprot
Y505	Phosphorylation	Uniprot
K508	Ubiquitination	Uniprot
K516	Ubiquitination	Uniprot
K553	Ubiquitination	Uniprot
K558	Ubiquitination	Uniprot
T559	Phosphorylation	Uniprot
K574	Ubiquitination	Uniprot
K575	Ubiquitination	Uniprot
K585	Ubiquitination	Uniprot
K586	Ubiquitination	Uniprot
T623	Phosphorylation	Uniprot
S658	Phosphorylation	Uniprot
K743	Ubiquitination	Uniprot
S779	Phosphorylation	Uniprot
S783	Phosphorylation	Uniprot
S785	Phosphorylation	Uniprot
S787	Phosphorylation	Uniprot
K793	Ubiquitination	Uniprot
K836	Ubiquitination	Uniprot
K842	Ubiquitination	Uniprot
K845	Ubiquitination	Uniprot

Research Backgrounds

P45974_UBP5_HUMAN

Function:

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Subunit Structure:

Interacts with TRIML1.

Family&Domains:

The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.

Belongs to the peptidase C19 family.

Restrictive clause

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USP5 Antibody - #DF12553