Product: ARID3A Antibody
Catalog: DF12558
Description: Rabbit polyclonal antibody to ARID3A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 75 kDa; 63kD(Calculated).
Uniprot: Q99856
RRID: AB_2845520

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ARID3A Antibody detects endogenous levels of total ARID3A.
RRID:
AB_2845520
Cite Format: Affinity Biosciences Cat# DF12558, RRID:AB_2845520.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARI3A_HUMAN; ARID domain containing 3A; ARID domain-containing protein 3A; ARID3A; AT rich interactive domain 3A (BRIGHT- like) protein; AT rich interactive domain 3A (BRIGHT-like); AT rich interactive domain-containing protein 3A; AT-rich interactive domain-containing protein 3A; B cell regulator of IgH transcription; B-cell regulator of IgH transcription; Bright; dead ringer like 1; dead ringer, Drosophila, homolog-like 1; Dead ringer-like protein 1; DRIL1; DRIL3; DRX; E2F binding protein 1; E2F-binding protein 1; E2FBP1; Homo sapiens AT rich interactive domain 3A (BRIGHT-like);

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99856 ARI3A_HUMAN:

Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.

Sequence:
MKLQAVMETLLQRQQRARQELEARQQLPPDPPAAPPGRARAAPDEDREPESARMQRAQMAALAAMRAAAAGLGHPASPGGSEDGPPGSEEEDAAREGTPGSPGRGREGPGEEHFEDMASDEDMKPKWEEEEMEEDLGEDEEEEEEDYEDEEEEEDEEGLGPPGPASLGTTALFPRKAQPPQAFRGDGVPRVLGGQERPGPGPAHPGGAAHVAPQLQPPDHGDWTYEEQFKQLYELDGDPKRKEFLDDLFSFMQKRGTPVNRIPIMAKQVLDLFMLYVLVTEKGGLVEVINKKLWREITKGLNLPTSITSAAFTLRTQYMKYLYPYECEKRGLSNPNELQAAIDSNRREGRRQSFGGSLFAYSPGGAHGMLSSPKLPVSSLGLAASTNGSSITPAPKIKKEEDSAIPITVPGRLPVSLAGHPVVAAQAAAVQAAAAQAAVAAQAAALEQLREKLESAEPPEKKMALVADEQQRLMQRALQQNFLAMAAQLPMSIRINSQASESRQDSAVNLTGTNGSNSISMSVEINGIMYTGVLFAQPPAPTPTSAPNKGGGGGGGSSSNAGGRGGNTGTSGGQAGPAGLSTPSTSTSNNSLP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
50
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99856 As Substrate

Site PTM Type Enzyme
S77 Phosphorylation
S81 Phosphorylation
S88 Phosphorylation
T98 Phosphorylation
S101 Phosphorylation
S119 Phosphorylation
K240 Ubiquitination
Y276 Phosphorylation
S309 Phosphorylation
K329 Ubiquitination
S353 Phosphorylation
S357 Phosphorylation
Y361 Phosphorylation
S362 Phosphorylation
K398 Sumoylation
T408 Phosphorylation
K452 Sumoylation
K452 Ubiquitination
K462 Sumoylation
S492 Phosphorylation
T568 Phosphorylation
T582 Phosphorylation
S584 Phosphorylation
S591 Phosphorylation

Research Backgrounds

Function:

Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between nucleus and cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.

Subunit Structure:

Homodimer. Heterodimer with ARID3B. Interacts with E2F1. Interacts with GTF2I and BTK.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.