CBFA2T3 Antibody - #DF12575
| Product: | CBFA2T3 Antibody |
| Catalog: | DF12575 |
| Description: | Rabbit polyclonal antibody to CBFA2T3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Chicken |
| Mol.Wt.: | 65 kDa; 71kD(Calculated). |
| Uniprot: | O75081 |
| RRID: | AB_2845537 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12575, RRID:AB_2845537.
Fold/Unfold
Cbfa2t3; Ccore binding factor runt domain alpha subunit 2 translocated to 3; ETO2; hMTG16; hMTG16a; hMTG16b; MTG16; MTG16_HUMAN; MTG8 related gene 2; MTG8 related protein MTG16a; MTG8 related protein MTG16b; MTG8-related protein 2; MTGR2; Myeloid translocation gene 8 and 16b; Myeloid translocation gene on chromosome 16 protein; Protein CBFA2T3; Zinc finger MYND domain-containing protein 4; ZMYND4;
Immunogens
Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).
- O75081 MTG16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPASRLRDRAASSASGSTCGSMSQTHPVLESGLLASAGCSAPRGPRKGGPAPVDRKAKASAMPDSPAEVKTQPRSTPPSMPPPPPAASQGATRPPSFTPHTHREDGPATLPHGRFHGCLKWSMVCLLMNGSSHSPTAINGAPCTPNGFSNGPATSSTASLSTQHLPPACGARQLSKLKRFLTTLQQFGSDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQTPAQYLAQHEQLLLDASASSPIDSSELLLEVNENGKRRTPDRTKENGSDRDPLHPEHLSKRPCTLNPAQRYSPSNGPPQPTPPPHYRLEDIAMAHHFRDAYRHPDPRELRERHRPLVVPGSRQEEVIDHKLTEREWAEEWKHLNNLLNCIMDMVEKTRRSLTVLRRCQEADREELNHWARRYSDAEDTKKGPAPAAARPRSSSAGPEGPQLDVPREFLPRTLTGYVPEDIWRKAEEAVNEVKRQAMSELQKAVSDAERKAHELITTERAKMERALAEAKRQASEDALTVINQQEDSSESCWNCGRKASETCSGCNAARYCGSFCQHRDWEKHHHVCGQSLQGPTAVVADPVPGPPEAAHSLGPSLPVGAASPSEAGSAGPSRPGSPSPPGPLDTVPR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75081 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| K47 | Methylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| Y327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| S578 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| T650 | Phosphorylation | Uniprot |
Research Backgrounds
Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity). Plays a role in granulocyte differentiation.
Isoform 2 functions as an A-kinase-anchoring protein.
Nucleus>Nucleolus.
Note: The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm.
Nucleus>Nucleoplasm. Golgi apparatus membrane.
Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).
Homooligomer. Homotetramerization is mediated by nervy homology region 2 (NRH2) (By similarity). Can interact with RUNX1T1 and CBFA2T2; heterotetramerization between members of the CBFA2T family is proposed. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3 (By similarity). Interacts with ERBB4, HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, NCOR1, NCOR2, and ZNF652. According to may not interact with HDAC6. Interacts with PLXNA1, PLXNA3 and PRKAR1A. Isoform 2 interacts with PRKAR2A, PDE7A and probably PDE4A. Interacts with ZBTB4, ZBTB38 and ZBTB33. Interacts with HIF1A and EGLN1. Interacts with the AML1-MTG8/ETO fusion protein.
Nervy homology region 2 (NHR2) mediates homo- and possibly heterotypic oligomerization by forming a four-helix bundle tetrameric structure.
Belongs to the CBFA2T family.
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