CBLL1 Antibody | Affinity Biosciences

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CBLL1 Antibody - Western blot analysis of extracts from MCF-7 cells, using CBLL1 Antibody.

Product:	CBLL1 Antibody
Catalog:	DF12576
Description:	Rabbit polyclonal antibody to CBLL1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	56 kDa; 55kD(Calculated).
Uniprot:	Q75N03
RRID:	AB_2845538

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

CBLL1 Antibody detects endogenous levels of total CBLL1.

RRID:

AB_2845538
Cite Format: Affinity Biosciences Cat# DF12576, RRID:AB_2845538.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

c-Cbl-like protein 1; CasBrM (murine) ecotropic retroviral transforming sequencelike 1; Casitas B-lineage lymphoma transforming sequence-like protein 1; Casitas B-lineage lymphoma-like 1; Casitas B-lineage lymphoma-transforming sequence-like protein 1; cbll1; E3 ubiquitin-protein ligase Hakai; Ecadherin binding protein E7; FLJ23109; HAKAI; HAKAI_HUMAN; MGC163401; MGC163403; OTTHUMP00000206720; OTTHUMP00000206722; OTTHUMP00000206724; RING finger protein 188; RNF188;

Immunogens

Immunogen:

Uniprot:

Q75N03(HAKAI_HUMAN) >>Visit HPA database.

Gene(ID):

CBLL1(79872)

Sequence:

Q75N03 HAKAI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDHTDNELQGTNSSGSLGGLDVRRRIPIKLISKQANKAKPAPRTQRTINRMPAKAPPGDEEGFDYNEEERYDCKGGELFANQRRFPGHLFWDFQINILGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQCTRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEIPERFIMPPDKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQPHEDIRAPPAELSMAPPPPRSVSQETFRISTRKHSNLITVPIQDDSNSGAREPPPPAPAPAHHHPEYQGQPVVSHPHHIMPPQQHYAPPPPPPPPISHPMPHPPQAAGTPHLVYSQAPPPPMTSAPPPITPPPGHIIAQMPPYMNHPPPGPPPPQHGGPPVTAPPPHHYNPNSLPQFTEDQGTLSPPFTQPGGMSPGIWPAPRGPPPPPRLQGPPSQTPLPGPHHPDQTRYRPYYQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q75N03 As Substrate

Q75N03

Site	PTM Type	Source
K29	Acetylation	Uniprot
K29	Methylation	Uniprot
K29	Ubiquitination	Uniprot
K33	Ubiquitination	Uniprot
T44	Phosphorylation	Uniprot
K74	Ubiquitination	Uniprot
Y119	Phosphorylation	Uniprot
Y176	Phosphorylation	Uniprot
R192	Methylation	Uniprot
T198	Phosphorylation	Uniprot
S201	Phosphorylation	Uniprot
R219	Methylation	Uniprot
S278	Phosphorylation	Uniprot
T281	Phosphorylation	Uniprot
R283	Methylation	Uniprot
S285	Phosphorylation	Uniprot
S290	Phosphorylation	Uniprot
R458	Methylation	Uniprot
R465	Methylation	Uniprot
R485	Methylation	Uniprot

Research Backgrounds

Q75N03_HAKAI_HUMAN

Function:

E3 ubiquitin-protein ligase that mediates ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1 (By similarity). Targets CDH1 for endocytosis and degradation (By similarity). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Its function in the WMM complex is unknown.

PTMs:

Phosphorylated on tyrosine residues.

Subcellular Location:

Nucleus speckle. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.

Subunit Structure:

Homodimer (By similarity). Interacts with tyrosine-phosphorylated SRC substrates (By similarity). Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM. The MAC subcomplex is composed of METTL3 and METTL14. The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B). Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3.

Family&Domains:

The HYB domain forms a phosphotyrosine-binding pocket upon dimerization, and mediates as well the recognition of its flanking acidic amino acids.

Belongs to the Hakai family.

Restrictive clause

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CBLL1 Antibody - #DF12576