CCDC120 Antibody - #DF12577

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Product: CCDC120 Antibody
Catalog: DF12577
Description: Rabbit polyclonal antibody to CCDC120
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 68 kDa; 68kD(Calculated).
Uniprot: Q96HB5
RRID: AB_2845539

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CCDC120 Antibody detects endogenous levels of total CCDC120.
RRID:
AB_2845539
Cite Format: Affinity Biosciences Cat# DF12577, RRID:AB_2845539.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA030924; CC120_HUMAN; CCDC120; coiled coil domain containing 120; coiled coil domain containing protein 120; Coiled-coil domain-containing protein 120; DXImx50e; JM11; RGD1561596; RP23-109E24.5; Sfc25;

Immunogens

Immunogen:
Uniprot:

Q96HB5(CC120_HUMAN) >>Visit HPA database.

Gene(ID):
CCDC120(90060)
Sequence:
MEVKGQLISSPTFNAPAALFGEAAPQVKSERLRGLLDRQRTLQEALSLKLQELRKVCLQEAELTGQLPPECPLEPGERPQLVRRRPPTARAYPPPHPNQAHHSLCPAEELALEALEREVSVQQQIAAAARRLALAPDLSTEQRRRRRQVQADALRRLHELEEQLRDVRARLGLPVLPLPQPLPLSTGSVITTQGVCLGMRLAQLSQEDVVLHSESSSLSESGASHDNEEPHGCFSLAERPSPPKAWDQLRAVSGGSPERRTPWKPPPSDLYGDLKSRRNSVASPTSPTRSLPRSASSFEGRSVPATPVLTRGAGPQLCKPEGLHSRQWSGSQDSQMGFPRADPASDRASLFVARTRRSNSSEALLVDRAAGGGAGSPPAPLAPSASGPPVCKSSEVLYERPQPTPAFSSRTAGPPDPPRAARPSSAAPASRGAPRLPPVCGDFLLDYSLDRGLPRSGGGTGWGELPPAAEVPGPLSRRDGLLTMLPGPPPVYAADSNSPLLRTKDPHTRATRTKPCGLPPEAAEGPEVHPNPLLWMPPPTRIPSAGERSGHKNLALEGLRDWYIRNSGLAAGPQRRPVLPSVGPPHPPFLHARCYEVGQALYGAPSQAPLPHSRSFTAPPVSGRYGGCFY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96HB5 As Substrate

Site PTM Type Enzyme
T88 Phosphorylation
S256 Phosphorylation
T261 Phosphorylation
S268 Phosphorylation
Y271 Phosphorylation
S280 Phosphorylation
S283 Phosphorylation
T285 Phosphorylation
S286 Phosphorylation
T288 Phosphorylation
S290 Phosphorylation
S294 Phosphorylation
S302 Phosphorylation
T306 Phosphorylation
R311 Methylation
S329 Phosphorylation
S331 Phosphorylation
S334 Phosphorylation
S358 Phosphorylation
S360 Phosphorylation
S361 Phosphorylation
S376 Phosphorylation
S393 Phosphorylation
Y398 Phosphorylation
T404 Phosphorylation
S409 Phosphorylation
S498 Phosphorylation
Y602 Phosphorylation
R614 Methylation
S615 Phosphorylation

Research Backgrounds

Function:

Centriolar protein required for centriole subdistal appendage assembly and microtubule anchoring in interphase cells. Together with CCDC68, cooperate with subdistal appendage components ODF2, NIN and CEP170 for hierarchical subdistal appendage assembly. Recruits NIN and CEP170 to centrosomes. Also required for neurite growth. Localizes CYTH2 to vesicles to allow its transport along neurites, and subsequent ARF6 activation and neurite growth.

PTMs:

Ubiquitinated; interaction with CYTH2 may prevent ubiquitination.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm. Cell projection>Neuron projection. Cell projection>Growth cone. Endosome.
Note: Localizes to the subdistal appendages of mother centrioles and proximal ends of both centrioles in interphase cells (PubMed:28422092). Recruited to subdistal appendages by ODF2 (PubMed:28422092). In differentiating neuroblastoma cells, colocalizes with CYTH2 in both neurite shaft and growth cone areas (PubMed:25326380). Partially colocalizes with endosomes along neurites in differentiating neuroblastoma cells (PubMed:25326380).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with NIN and CEP170; leading to recruit them to centrosomes. Directly interacts with CYTH2; this interaction stabilizes CCDC120, possibly by preventing ubiquitination.

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