Product: DCUN1D5 Antibody
Catalog: DF12595
Description: Rabbit polyclonal antibody to DCUN1D5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 28 kDa, 37 kDa; 28kD(Calculated).
Uniprot: Q9BTE7
RRID: AB_2845557

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DCUN1D5 Antibody detects endogenous levels of total DCUN1D5.
RRID:
AB_2845557
Cite Format: Affinity Biosciences Cat# DF12595, RRID:AB_2845557.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DCN1, defective in cullin neddylation 1, domain containing 5; DCN1-like protein 5; DCUN1 domain-containing protein 5; defective in cullin neddylation protein 1-like protein 5; RP11-690D19.3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSKKCLAWFYEYAGPDEVVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSLQCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFARDKDQRSLDIDTAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHADLSNYDEDGAWPVLLDEFVEWQKVRQTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
89
Zebrafish
83
Xenopus
78
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BTE7 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
S9 Phosphorylation
K23 Methylation
K23 Ubiquitination
K24 Methylation
K26 Ubiquitination
S41 Phosphorylation
S48 Phosphorylation
K49 Ubiquitination
K103 Ubiquitination
K108 Ubiquitination
K120 Ubiquitination
K124 Ubiquitination
K139 Ubiquitination
K163 Ubiquitination
S164 Phosphorylation
K194 Ubiquitination

Research Backgrounds

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.