GPNMB Antibody | Affinity Biosciences

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IF/ICC

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GPNMB Antibody - Western blot analysis of extracts from A375 cells, using GPNMB Antibody.

GPNMB Antibody - Western blot analysis of extracts from K562, using GPNMB Antibody.

GPNMB Antibody - DF12621 staining Hela cells by IF/ICC.

Product:	GPNMB Antibody
Catalog:	DF12621
Description:	Rabbit polyclonal antibody to GPNMB
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Horse
Mol.Wt.:	68 kDa; 64kD(Calculated).
Uniprot:	Q14956
RRID:	AB_2845583

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Horse(86%)

Clonality:

Polyclonal

Specificity:

GPNMB Antibody detects endogenous levels of total GPNMB.

RRID:

AB_2845583
Cite Format: Affinity Biosciences Cat# DF12621, RRID:AB_2845583.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glycoprotein (transmembrane) nmb; Glycoprotein nmb; Glycoprotein nmb like protein; Gpnmb; GPNMB_HUMAN; HGFIN; NMB; Osteoactivin; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB;

Immunogens

Immunogen:

Uniprot:

Q14956(GPNMB_HUMAN) >>Visit HPA database.

Gene(ID):

GPNMB(10457)

Expression:

Q14956 GPNMB_HUMAN:

Widely expressed, but very low expression, if any, in the brain (PubMed:12609765, PubMed:16609006). Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level) (PubMed:29336782). Expressed in peripheral blood, but not bone marrow mononuclear cells (PubMed:12609765). Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level) (PubMed:16489096). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme (PubMed:7814155, PubMed:16489096, PubMed:16609006).

Sequence:

Q14956 GPNMB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MECLYYFLGFLLLAARLPLDAAKRFHDVLGNERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNSSDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLATTLKSYDSNTPGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVPDRDPASPLRMANSALISVGCLAIFVTVISLLVYKKHKEYNPIENSPGNVVRSKGLSVFLNRAKAVFFPGNQEKDPLLKNQEFKGVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Bovine

Pig

Sheep

Xenopus

Rabbit

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14956 As Substrate

Q14956

Site	PTM Type	Source
S35	Phosphorylation	Uniprot
Y37	Phosphorylation	Uniprot
N200	N-Glycosylation	Uniprot
Y220	Phosphorylation	Uniprot
Y230	Phosphorylation	Uniprot
N249	N-Glycosylation	Uniprot
N275	N-Glycosylation	Uniprot
N296	N-Glycosylation	Uniprot
N300	N-Glycosylation	Uniprot
N306	N-Glycosylation	Uniprot
S499	Phosphorylation	Uniprot
S503	Phosphorylation	Uniprot
K523	Ubiquitination	Uniprot
Y525	Phosphorylation	Uniprot
S531	Phosphorylation	Uniprot
K539	Ubiquitination	Uniprot
S542	Phosphorylation	Uniprot
K549	Ubiquitination	Uniprot
K559	Ubiquitination	Uniprot
K564	Ubiquitination	Uniprot

Research Backgrounds

Q14956_GPNMB_HUMAN

Function:

Could be a melanogenic enzyme.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Melanosome membrane>Single-pass type I membrane protein. Early endosome membrane>Single-pass type I membrane protein.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:

Widely expressed, but very low expression, if any, in the brain. Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level). Expressed in peripheral blood, but not bone marrow mononuclear cells. Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme.

Family&Domains:

Belongs to the PMEL/NMB family.

References

1). Mechanistic study of the regulation of mitochondrial function by the GPNMB/Nrf2/NF-κB signaling pathway mediated by Quzhi Tang to alleviate chondrocyte senescence. Journal of ethnopharmacology, 2024 (PubMed: 39617085) [IF=5.4]

2). Mechanistic study of the regulation of mitochondrial function by the GPNMB/Nrf2/NF-κB signaling pathway mediated by Quzhi Tang to alleviate chondrocyte senescence. Journal of ethnopharmacology, 2024 (PubMed: 39617085) [IF=4.8]

Restrictive clause

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GPNMB Antibody - #DF12621