HELLS Antibody - #DF12631

(3)  
Product: HELLS Antibody
Catalog: DF12631
Description: Rabbit polyclonal antibody to HELLS
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 97 kDa; 97kD(Calculated).
Uniprot: Q9NRZ9
RRID: AB_2845593

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(83%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
HELLS Antibody detects endogenous levels of total HELLS.
RRID:
AB_2845593
Cite Format: Affinity Biosciences Cat# DF12631, RRID:AB_2845593.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ10339; Helicase lymphoid specific; Hells; HELLS_HUMAN; LSH; Lymphoid specific helicase; Lymphoid-specific helicase; Nbla10143; PASG; Proliferation associated SNF2 like protein; Proliferation-associated SNF2-like protein; SWI/SNF2 related matrix associated actin dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6;

Immunogens

Immunogen:
Uniprot:

Q9NRZ9(HELLS_HUMAN) >>Visit HPA database.

Gene(ID):
HELLS(3070)
Expression:
Q9NRZ9 HELLS_HUMAN:

Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias.

Sequence:
MPAERPAGSGGSEAPAMVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLERKKESLKVKKGKNSIDASEEKPVMRKKRGREDESYNISEVMSKEEILSVAKKNKKENEDENSSSTNLCVEDLQKNKDSNSIIKDRLSETVRQNTKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPDIPTMLYHGTQEERQKLVRNIYKRKGTLQIHPVVITSFEIAMRDRNALQHCYWKYLIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIANMFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
92
Dog
83
Zebrafish
73
Xenopus
71
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRZ9 As Substrate

Site PTM Type Enzyme
K74 Ubiquitination
K80 Ubiquitination
K85 Ubiquitination
K99 Acetylation
K103 Acetylation
K104 Acetylation
S106 Phosphorylation
S115 Phosphorylation
S119 Phosphorylation
K122 Acetylation
K122 Sumoylation
K122 Ubiquitination
K144 Ubiquitination
S149 Phosphorylation
K152 Ubiquitination
K175 Ubiquitination
K177 Ubiquitination
K184 Ubiquitination
S188 Phosphorylation
T190 Phosphorylation
K196 Ubiquitination
K204 Sumoylation
K204 Ubiquitination
K216 Ubiquitination
K409 Sumoylation
Y469 Phosphorylation
S473 Phosphorylation
K475 Sumoylation
K475 Ubiquitination
T481 Phosphorylation
T487 Phosphorylation
T499 Phosphorylation
S503 Phosphorylation
T505 Phosphorylation
K514 Ubiquitination
S515 Phosphorylation
Y518 Phosphorylation
S519 Phosphorylation
K520 Ubiquitination
K530 Ubiquitination
K569 Methylation
K600 Ubiquitination
K612 Ubiquitination
S648 Phosphorylation
S650 Phosphorylation
Y651 Phosphorylation
S652 Phosphorylation
T714 Phosphorylation
K715 Ubiquitination
K731 Ubiquitination
K741 Ubiquitination
K744 Ubiquitination
K749 Ubiquitination
K753 Methylation
K753 Ubiquitination
S757 Phosphorylation
K763 Ubiquitination
K769 Ubiquitination
K776 Ubiquitination
K785 Ubiquitination
K790 Ubiquitination
K795 Ubiquitination
R803 Methylation
S813 Phosphorylation
K817 Ubiquitination
K819 Ubiquitination
S829 Phosphorylation
S832 Phosphorylation
S833 Phosphorylation

Research Backgrounds

Function:

Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis (By similarity).

Subcellular Location:

Nucleus.
Note: Closely associated with pericentric heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

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