Product: HMG20A Antibody
Catalog: DF12635
Description: Rabbit polyclonal antibody to HMG20A
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 50 kDa; 40kD(Calculated).
Uniprot: Q9NP66
RRID: AB_2845597

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
HMG20A Antibody detects endogenous levels of total HMG20A.
RRID:
AB_2845597
Cite Format: Affinity Biosciences Cat# DF12635, RRID:AB_2845597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ10739; High mobility group 20A; High mobility group protein 20A; HM20A_HUMAN; HMG box containing protein 20A; HMG box domain containing 1; HMG box-containing protein 20A; HMG domain protein HMGX1; HMG domain-containing protein 1; HMG domain-containing protein HMGX1; hmg20a; HMGX1; HMGXB1; Hypothetical protein FLJ10739; iBRAF; Inhibitor of BRAF35;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NP66 HM20A_HUMAN:

Ubiquitous.

Sequence:
MENLMTSSTLPPLFADEDGSKESNDLATTGLNHPEVPYSSGATSSTNNPEFVEDLSQGQLLQSESSNAAEGNEQRHEDEQRSKRGGWSKGRKRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEKLEVDVIQERSRNTVLQQHLETLRQVLTSSFASMPLPGSGETPTVDTIDSYMNRLHSIILANPQDNENFIATVREVVNRLDR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
75
Zebrafish
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NP66 As Substrate

Site PTM Type Enzyme
T6 Phosphorylation
S20 Phosphorylation
S100 Phosphorylation
S105 Phosphorylation
T108 Phosphorylation
Y152 Phosphorylation
Y163 Phosphorylation
K165 Ubiquitination
Y170 Phosphorylation
K172 Ubiquitination
T173 Phosphorylation
Y176 Phosphorylation
K177 Ubiquitination
K230 Ubiquitination
K255 Ubiquitination
T287 Phosphorylation

Research Backgrounds

Function:

Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase KMT2A/MLL1 and consequent increased methylation of histone H3 lysine 4 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Restrictive clause

 

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For Research Use Only.
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