MITD1 Antibody | Affinity Biosciences

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MITD1 Antibody - Western blot analysis of extracts from NIH/3T3 cells, using MITD1 Antibody.

Product:	MITD1 Antibody
Catalog:	DF12659
Description:	Rabbit polyclonal antibody to MITD1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	29 kDa; 29kD(Calculated).
Uniprot:	Q8WV92
RRID:	AB_2845621

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

MITD1 Antibody detects endogenous levels of total MITD1.

RRID:

AB_2845621
Cite Format: Affinity Biosciences Cat# DF12659, RRID:AB_2845621.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q8WV92(MITD1_HUMAN) >>Visit HPA database.

Gene(ID):

MITD1(129531)

Sequence:

Q8WV92 MITD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWIEDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSHGVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDIFHKKHTKNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Bovine

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WV92 As Substrate

Q8WV92

Site	PTM Type	Source
K20	Ubiquitination	Uniprot
Y38	Phosphorylation	Uniprot
K67	Ubiquitination	Uniprot
Y68	Phosphorylation	Uniprot
Y78	Phosphorylation	Uniprot

Research Backgrounds

Q8WV92_MITD1_HUMAN

Function:

Required for efficient abscission at the end of cytokinesis, together with components of the ESCRT-III complex.

Subcellular Location:

Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Midbody. Membrane>Peripheral membrane protein>Cytoplasmic side.
Note: During cytokinesis, recruited to the midbody via interaction with CHMP1A. Interacts with membranes enriched in phosphoinositides.

Subunit Structure:

Homodimer. Interacts (via MIT domain) with CHMP1A, CHMP1B, CHMP2A and IST1.

Family&Domains:

The C-terminal domain interacts with lipid membranes containing acidic phosphoinositides and is required for location at the midbody.

The MIT domain interacts with the MIT-interacting motifs of several components of the ESCRT-III complex.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MITD1 Antibody - #DF12659