NOSTRIN Antibody - #DF12673

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Product: NOSTRIN Antibody
Catalog: DF12673
Description: Rabbit polyclonal antibody to NOSTRIN
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 58 kDa; 58kD(Calculated).
Uniprot: Q8IVI9
RRID: AB_2845635

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
NOSTRIN Antibody detects endogenous levels of total NOSTRIN.
RRID:
AB_2845635
Cite Format: Affinity Biosciences Cat# DF12673, RRID:AB_2845635.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BM247; BM247 homolog; DaIP2; eNOS-trafficking inducer; mDaIP2; MGC20702; Nitric oxide synthase traffic inducer; Nitric oxide synthase trafficker; NOSTN_HUMAN; Nostrin; RP23-431D4.2;

Immunogens

Immunogen:
Uniprot:

Q8IVI9(NOSTN_HUMAN) >>Visit HPA database.

Gene(ID):
NOSTRIN(115677)
Expression:
Q8IVI9 NOSTN_HUMAN:

Expressed at highest levels in heart, kidney, placenta and lung, and at lowest levels in brain, thymus and spleen. Present in vascular endothelial cells and placenta. Over-expressed in placenta from women with pre-eclampsia (at protein level).

Sequence:
MRDPLTDCPYNKVYKNLKEFSQNGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSSAWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFQLVESSKQSMTEKEKRKLLNKLTKSTEKLEKEDENYYQKNMAGYSTRLKWENTLENCYQSILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIEKDIQAVMEETAILSTENKSEFLLTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASKDKEGLERMLKTYSSTSSFSDAKSQKDTAALMDENNLKLDLLEANSYKLSSMLAELEQRPQPSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASSGGQSNPGSSTPAPGAAQLSSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEGGWWFGSLNGKKGHFPAAYVEELPSNAGNTATKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Sheep
92
Pig
83
Dog
83
Rabbit
83
Zebrafish
67
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IVI9 As Substrate

Site PTM Type Enzyme
K113 Methylation
S114 Phosphorylation
S143 Phosphorylation
T144 Phosphorylation
T174 Phosphorylation
S176 Phosphorylation
T177 Phosphorylation
S277 Phosphorylation
Y330 Phosphorylation
S332 Phosphorylation
S334 Phosphorylation
T430 Phosphorylation
Y447 Phosphorylation
S448 Phosphorylation
S479 Phosphorylation

Research Backgrounds

Function:

Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle. Cytoplasm>Cytoskeleton.
Note: Enriched in selected actin structures (PubMed:16234328, PubMed:16376344).

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at highest levels in heart, kidney, placenta and lung, and at lowest levels in brain, thymus and spleen. Present in vascular endothelial cells and placenta. Over-expressed in placenta from women with pre-eclampsia (at protein level).

Subunit Structure:

Homotrimer. Interacts with DAB2 (By similarity). Interacts with NOS3, DNM2, WASL and CAV1.

Family&Domains:

The SH3 domain mediates interaction with NOS3, DNM2 and WASL.

The F-BAR domain is necessary for membrane targeting.

Restrictive clause

 

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