NXN Antibody | Affinity Biosciences

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NXN Antibody - Western blot analysis of extracts from HeLa cells, using NXN Antibody.

Product:	NXN Antibody
Catalog:	DF12679
Description:	Rabbit polyclonal antibody to NXN
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Dog, Xenopus
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	Q6DKJ4
RRID:	AB_2845641

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Rabbit(100%), Dog(90%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

NXN Antibody detects endogenous levels of total NXN.

RRID:

AB_2845641
Cite Format: Affinity Biosciences Cat# DF12679, RRID:AB_2845641.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ12614; NRX; nucleoredoxin 1; Nucleoredoxin; Nxn; NXN_HUMAN; OTTHUMP00000115356; TRG 4;

Immunogens

Immunogen:

Uniprot:

Q6DKJ4(NXN_HUMAN) >>Visit HPA database.

Gene(ID):

NXN(64359)

Sequence:

Q6DKJ4 NXN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGFLEELLGEKLVTGGGEEVDVHSLGARGISLLGLYFGCSLSAPCAQLSASLAAFYGRLRGDAAAGPGPGAGAGAAAEPEPRRRLEIVFVSSDQDQRQWQDFVRDMPWLALPYKEKHRKLKLWNKYRISNIPSLIFLDATTGKVVCRNGLLVIRDDPEGLEFPWGPKPFREVIAGPLLRNNGQSLESSSLEGSHVGVYFSAHWCPPCRSLTRVLVESYRKIKEAGQNFEIIFVSADRSEESFKQYFSEMPWLAVPYTDEARRSRLNRLYGIQGIPTLIMLDPQGEVITRQGRVEVLNDEDCREFPWHPKPVLELSDSNAAQLNEGPCLVLFVDSEDDGESEAAKQLIQPIAEKIIAKYKAKEEEAPLLFFVAGEDDMTDSLRDYTNLPEAAPLLTILDMSARAKYVMDVEEITPAIVEAFVNDFLAEKLKPEPI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Rabbit

100

Dog

Xenopus

Horse

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6DKJ4 As Substrate

Q6DKJ4

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
T289	Phosphorylation	Uniprot
K354	Ubiquitination	Uniprot

Research Backgrounds

Q6DKJ4_NXN_HUMAN

Function:

Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A) (By similarity).

Subcellular Location:

Cytoplasm>Cytosol. Nucleus.

Subunit Structure:

Associates with the phosphatase 2A holoenzyme. Interacts with PPP2CA; the interaction is direct. Interacts with DVL1 (via PDZ domain); the interaction is direct and regulated by oxidative stress (By similarity).

Family&Domains:

Belongs to the nucleoredoxin family.

Restrictive clause

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For Research Use Only.
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NXN Antibody - #DF12679