PIH1D1 Antibody - #DF12697
| Product: | PIH1D1 Antibody |
| Catalog: | DF12697 |
| Description: | Rabbit polyclonal antibody to PIH1D1 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 39 kDa; 32kD(Calculated). |
| Uniprot: | Q9NWS0 |
| RRID: | AB_2845658 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12697, RRID:AB_2845658.
Immunogens
Expressed at low levels in normal mammary epithelial cells (at protein level) (PubMed:24036451). Highest expression in lung, leukocyte and placenta. Expressed at lower levels in brain, prostate, colon, heart, small intestine, liver, ovary, pancreas, skeletal muscle, spleen, testis and thymus (PubMed:21078300).
- Q9NWS0 PIHD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANPKLLGMGLSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEVDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLPVPS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NWS0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.
Nucleus.
Expressed at low levels in normal mammary epithelial cells (at protein level). Highest expression in lung, leukocyte and placenta. Expressed at lower levels in brain, prostate, colon, heart, small intestine, liver, ovary, pancreas, skeletal muscle, spleen, testis and thymus.
Component of the R2TP complex composed at least of PIHD1, RUVBL1, RUVBL2 and RPAP3. Interacts with phosphorylated TELO2 and mediates interaction of TELO2 with the R2TP complex. Interacts with phosphorylated ECD, EFTUD2/SNRP116, RPB1 and UBR5 and with RPB1 in a phosphorylation-independent manner. Interacts with the core C/D box snoRNP particle components NOP58 and FBL and with RUVBL1/TIP49. Interacts with RPAP3 and WDR92. Interacts with histone H4 and with SWI/SNF complex member SMARCB1/SNF5. Interacts with the mTORC1 complex member RPTOR. Interacts with MSL1 (By similarity).
The N-terminal region is required for binding to phosphorylated substrates while the C-terminal region binds to the other R2TP complex components.
Belongs to the PIH1 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.