RIC8A Antibody - #DF12722

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Product: RIC8A Antibody
Catalog: DF12722
Description: Rabbit polyclonal antibody to RIC8A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: Q9NPQ8
RRID: AB_2845683

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
RIC8A Antibody detects endogenous levels of total RIC8A.
RRID:
AB_2845683
Cite Format: Affinity Biosciences Cat# DF12722, RRID:AB_2845683.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC104517; MGC131931; MGC148073; MGC148074; Protein Ric-8A; resistance to inhibitors of cholinesterase 8 homolog A (C. elegans); RIC8; RIC8A; RIC8A_HUMAN; Synembryn-A;

Immunogens

Immunogen:
Uniprot:

Q9NPQ8(RIC8A_HUMAN) >>Visit HPA database.

Gene(ID):
RIC8A(60626)
Sequence:
MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPPSHRVIWLQSVRILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKCLCNLVLSSPVAQMLAAEARLVVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQQLFQELKGVRLLTDTLELTLGVTPEGNPPPTLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
92
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Chicken
64
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPQ8 As Substrate

Site PTM Type Enzyme
T12 Phosphorylation
Y25 Phosphorylation
S30 Phosphorylation
S32 Phosphorylation
S74 Phosphorylation
T193 Phosphorylation
T195 Phosphorylation
T199 Phosphorylation
T203 Phosphorylation
T324 Phosphorylation
K328 Ubiquitination
K353 Ubiquitination
K409 Ubiquitination
Y435 Phosphorylation
S436 Phosphorylation
T441 Phosphorylation
T443 Phosphorylation
Y446 Phosphorylation
K450 Ubiquitination
S452 Phosphorylation
T485 Phosphorylation
K489 Ubiquitination
S491 Phosphorylation
S502 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation
S528 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma. Interacts (via C-terminus) with RGS14; the interaction stimulates the dissociation of the complex between RGS14 and the active GTP-bound form of GNAI1 (By similarity).

Family&Domains:

Belongs to the synembryn family.

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