RLIM Antibody - #DF12723
| Product: | RLIM Antibody |
| Catalog: | DF12723 |
| Description: | Rabbit polyclonal antibody to RLIM |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 75 kDa; 69kD(Calculated). |
| Uniprot: | Q9NVW2 |
| RRID: | AB_2845684 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12723, RRID:AB_2845684.
Fold/Unfold
E3 ubiquitin-protein ligase RLIM; LIM domain-interacting RING finger protein; R-LIM; Renal carcinoma antigen NY-REN-43; RING finger LIM domain-binding protein; RING finger protein 12; RLIM; RNF12; RNF12_HUMAN;
Immunogens
- Q9NVW2 RNF12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NVW2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| R117 | Methylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| R360 | Methylation | Uniprot | |
| R364 | Methylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S468 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K582 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot |
Research Backgrounds
E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.
Nucleus.
Expressed in many tissues.
Interacts with LIM/homeobox factors such as LHX3. Interacts with LDB1, LDB2 and SIN3A (By similarity). Interacts with LIMK1 (By similarity). Interacts (via N-terminus) with TERF1. Interacts (via C-terminus) with ESR1.
Belongs to the RNF12 family.
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