SAMM50 Antibody - #DF12729

(2)  
Product: SAMM50 Antibody
Catalog: DF12729
Description: Rabbit polyclonal antibody to SAMM50
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
Mol.Wt.: 52 kDa; 52kD(Calculated).
Uniprot: Q9Y512
RRID: AB_2845690

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
SAMM50 Antibody detects endogenous levels of total SAMM50.
RRID:
AB_2845690
Cite Format: Affinity Biosciences Cat# DF12729, RRID:AB_2845690.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CGI 51; CGI-51; FLJ35825; FLJ42905; FLJ99036; OMP85; SAM50; SAM50_HUMAN; Samm50; sorting and assembly machinery 50kDa; sorting and assembly machinery component 50 homolog (S. cerevisiae); Sorting and assembly machinery component 50 homolog; sorting and assembly machinery component 50, S. cerevisiae, homolog of; TOB55; Transformation-related gene 3 protein; TRG-3; YNL026W;

Immunogens

Immunogen:
Uniprot:

Q9Y512(SAM50_HUMAN) >>Visit HPA database.

Gene(ID):
SAMM50(25813)
Sequence:
MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSIRGFSMHSIGPQSEGDYLGGEAYWAGGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
92
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y512 As Substrate

Site PTM Type Enzyme
K36 Ubiquitination
K43 Ubiquitination
K59 Ubiquitination
C65 S-Nitrosylation
K72 Ubiquitination
K74 Ubiquitination
K178 Acetylation
K178 Ubiquitination
S189 Phosphorylation
Y215 Phosphorylation
S216 Phosphorylation
K221 Ubiquitination
K227 Acetylation
K227 Ubiquitination
S239 Phosphorylation
S243 Phosphorylation
K255 Methylation
S269 Phosphorylation
S270 Phosphorylation
K297 Acetylation
K412 Ubiquitination
K417 Ubiquitination

Research Backgrounds

Function:

Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes. Required for the assembly of TOMM40 into the TOM complex.

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein. Cytoplasm. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with CHCHD3/MIC19.

Family&Domains:

Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.

Belongs to the SAM50/omp85 family.

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