SOX5 Antibody - #DF12747
(2)
| Product: | SOX5 Antibody |
| Catalog: | DF12747 |
| Description: | Rabbit polyclonal antibody to SOX5 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 70 kDa; 84kD(Calculated). |
| Uniprot: | P35711 |
| RRID: | AB_2845708 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
SOX5 Antibody detects endogenous levels of total SOX5.
RRID:
AB_2845708
Cite Format: Affinity Biosciences Cat# DF12747, RRID:AB_2845708.
Cite Format: Affinity Biosciences Cat# DF12747, RRID:AB_2845708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
L SOX5; MGC35153; Sex determining region Y box 5; SOX 5; SOX 5 protein; Sox5; SOX5 protein; SOX5_HUMAN; SRY (sex determining region Y) box 5; SRY box 5; Transcription factor SOX 5; Transcription factor SOX-5; Transcription factor SOX5;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P35711 SOX5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLTDPDLPQEFERMSSKRPASPYGEADGEVAMVTSRQKVEEEESDGLPAFHLPLHVSFPNKPHSEEFQPVSLLTQETCGHRTPTSQHNTMEVDGNKVMSSFAPHNSSTSPQKAEEGGRQSGESLSSTALGTPERRKGSLADVVDTLKQRKMEELIKNEPEETPSIEKLLSKDWKDKLLAMGSGNFGEIKGTPESLAEKERQLMGMINQLTSLREQLLAAHDEQKKLAASQIEKQRQQMELAKQQQEQIARQQQQLLQQQHKINLLQQQIQVQGQLPPLMIPVFPPDQRTLAAAAQQGFLLPPGFSYKAGCSDPYPVQLIPTTMAAAAAATPGLGPLQLQQLYAAQLAAMQVSPGGKLPGIPQGNLGAAVSPTSIHTDKSTNSPPPKSKDEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35711 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S21 | Phosphorylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| T413 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| K467 | Sumoylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| R549 | Methylation | Uniprot | |
| K595 | Acetylation | Uniprot | |
| K619 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro.
Subcellular Location:
Nucleus.
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