Product: SPATA22 Antibody
Catalog: DF12748
Description: Rabbit polyclonal antibody to SPATA22
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 41 kDa; 41kD(Calculated).
Uniprot: Q8NHS9
RRID: AB_2845709

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(83%), Bovine(100%), Horse(92%), Rabbit(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SPATA22 Antibody detects endogenous levels of total SPATA22.
RRID:
AB_2845709
Cite Format: Affinity Biosciences Cat# DF12748, RRID:AB_2845709.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NYD SP20; SPATA 22; SPATA22; Spermatogenesis associated 22; Spermatogenesis associated protein 22; Spermatogenesis-associated protein 22; SPT22_HUMAN; Testes development related NYD SP20; Testis development protein NYD SP20; Testis development protein NYD-SP20;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8NHS9 SPT22_HUMAN:

Highly expressed in adult testis.

Sequence:
MKRSLNENSARSTAGCLPVPLFNQKKRNRQPLTSNPLKDDSGISTPSDNYDFPPLPTDWAWEAVNPELAPVMKTVDTGQIPHSVSRPLRSQDSVFNSIQSNTGRSQGGWSYRDGNKNTSLKTWNKNDFKPQCKRTNLVANDGKNSCPVSSGAQQQKQLRIPEPPNLSRNKETELLRQTHSSKISGCTMRGLDKNSALQTLKPNFQQNQYKKQMLDDIPEDNTLKETSLYQLQFKEKASSLRIISAVIESMKYWREHAQKTVLLFEVLAVLDSAVTPGPYYSKTFLMRDGKNTLPCVFYEIDRELPRLIRGRVHRCVGNYDQKKNIFQCVSVRPASVSEQKTFQAFVKIADVEMQYYINVMNET

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Horse
92
Pig
83
Rabbit
83
Zebrafish
67
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NHS9 As Substrate

Site PTM Type Enzyme
S167 Phosphorylation
S238 Phosphorylation
S239 Phosphorylation
S244 Phosphorylation
S249 Phosphorylation
Y252 Phosphorylation
Y279 Phosphorylation
Y280 Phosphorylation

Research Backgrounds

Function:

Meiosis-specific protein required for homologous recombination in meiosis I.

Subcellular Location:

Chromosome.
Note: Localizes on meiotic chromosome axes. Accumulates on resected DNA. Localization is dependent on MEIOB (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in adult testis.

Subunit Structure:

Component of a multiprotein complex with MEIOB and SPATA22.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.