SPATA7 Antibody | Affinity Biosciences

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SPATA7 Antibody - Western blot analysis of extracts from PC-3 cells, using SPATA7 Antibody.

Product:	SPATA7 Antibody
Catalog:	DF12749
Description:	Rabbit polyclonal antibody to SPATA7
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	68 kDa; 68kD(Calculated).
Uniprot:	Q9P0W8
RRID:	AB_2845710

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

SPATA7 Antibody detects endogenous levels of total SPATA7.

RRID:

AB_2845710
Cite Format: Affinity Biosciences Cat# DF12749, RRID:AB_2845710.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp686D07199; epididymis secretory protein Li 296; HEL-S-296; HSD 3.1; HSD3; LCA3; MGC102934; SPATA 7; Spermatogenesis associated 7; Spermatogenesis associated protein 7; Spermatogenesis associated protein HSD3;

Immunogens

Immunogen:

Uniprot:

Q9P0W8(SPAT7_HUMAN) >>Visit HPA database.

Gene(ID):

SPATA7(55812)

Sequence:

Q9P0W8 SPAT7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDGSRRVRATSVLPRYGPPCLFKGHLSTKSNAFCTDSSSLRLSTLQLVKNHMAVHYNKILSAKAAVDCSVPVSVSTSIKYADQQRREKLKKELAQCEKEFKLTKTAMRANYKNNSKSLFNTLQKPSGEPQIEDDMLKEEMNGFSSFARSLVPSSERLHLSLHKSSKVITNGPEKNSSSSPSSVDYAASGPRKLSSGALYGRRPRSTFPNSHRFQLVISKAPSGDLLDKHSELFSNKQLPFTPRTLKTEAKSFLSQYRYYTPAKRKKDFTDQRIEAETQTELSFKSELGTAETKNMTDSEMNIKQASNCVTYDAKEKIAPLPLEGHDSTWDEIKDDALQHSSPRAMCQYSLKPPSTRKIYSDEEELLYLSFIEDVTDEILKLGLFSNRFLERLFERHIKQNKHLEEEKMRHLLHVLKVDLGCTSEENSVKQNDVDMLNVFDFEKAGNSEPNELKNESEVTIQQERQQYQKALDMLLSAPKDENEIFPSPTEFFMPIYKSKHSEGVIIQQVNDETNLETSTLDENHPSISDSLTDRETSVNVIEGDSDPEKVEISNGLCGLNTSPSQSVQFSSVKGDNNHDMELSTLKIMEMSIEDCPLDV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Dog

100

Rabbit

100

Bovine

Chicken

Xenopus

Pig

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P0W8 As Substrate

Q9P0W8

Site	PTM Type	Source
R8	Methylation	Uniprot
R15	Methylation	Uniprot
Y16	Phosphorylation	Uniprot
S39	Phosphorylation	Uniprot
K104	Ubiquitination	Uniprot
S144	Phosphorylation	Uniprot
S145	Phosphorylation	Uniprot
K174	Ubiquitination	Uniprot
S194	Phosphorylation	Uniprot
T206	Phosphorylation	Uniprot
K219	Ubiquitination	Uniprot
K228	Ubiquitination	Uniprot
K236	Ubiquitination	Uniprot
Y258	Phosphorylation	Uniprot
Y259	Phosphorylation	Uniprot
T289	Phosphorylation	Uniprot
K316	Ubiquitination	Uniprot
K333	Ubiquitination	Uniprot
Y467	Phosphorylation	Uniprot
K469	Ubiquitination	Uniprot

Research Backgrounds

Q9P0W8_SPAT7_HUMAN

Function:

Involved in the maintenance of both rod and cone photoreceptor cells (By similarity). It is required for recruitment and proper localization of RPGRIP1 to the photoreceptor connecting cilium (CC), as well as photoreceptor-specific localization of proximal CC proteins at the distal CC (By similarity). Maintenance of protein localization at the photoreceptor-specific distal CC is essential for normal microtubule stability and to prevent photoreceptor degeneration (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium axoneme. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton. Cell projection>Cilium>Photoreceptor outer segment.
Note: Localizes to the microtubule network.

Subunit Structure:

Found in a complex with CFAP410, NEK1 and SPATA7. Interacts with NEK1. Interacts with RPGRIP1. Interacts with RPGR (By similarity). Interacts with NPHP4 (By similarity). Interacts with NPHP1 (By similarity). Interacts with AHI1 (By similarity).

Restrictive clause

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SPATA7 Antibody - #DF12749