TDRKH Antibody - #DF12768

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Product: TDRKH Antibody
Catalog: DF12768
Description: Rabbit polyclonal antibody to TDRKH
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 67 kDa; 62kD(Calculated).
Uniprot: Q9Y2W6
RRID: AB_2845729

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(86%)
Clonality:
Polyclonal
Specificity:
TDRKH Antibody detects endogenous levels of total TDRKH.
RRID:
AB_2845729
Cite Format: Affinity Biosciences Cat# DF12768, RRID:AB_2845729.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2700091C21Rik; Putative RNA binding protein; RGD1311196; RP11-98D18.8; TDRD2; TDRD7; Tdrkh; TDRKH_HUMAN; Tudor and KH domain containing; Tudor and KH domain-containing protein; tudor domain containing 2; Tudor domain-containing protein 2;

Immunogens

Immunogen:
Uniprot:

Q9Y2W6(TDRKH_HUMAN) >>Visit HPA database.

Gene(ID):
TDRKH(11022)
Sequence:
MSTERTSWTSLSTIQKIALGLGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKGGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRARVLGTLENGNLDLYFVDFGDNGDCPLKDLRALRSDFLSLPFQAIECSLARIAPSGDQWEEEALDEFDRLTHCADWKPLVAKISSYVQTGISTWPKIYLYDTSNGKKLDIGLELVHKGYAIELPEDIEENRAVPDMLKDMATETDASLSTLLTETKKSSGEITHTLSCLSLSEAASMSGDDNLEDDYLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Rabbit
86
Pig
82
Dog
67
Zebrafish
30
Chicken
30
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2W6 As Substrate

Site PTM Type Enzyme
Y31 Phosphorylation
K65 Ubiquitination
K76 Ubiquitination
T90 Phosphorylation
K110 Ubiquitination
K112 Ubiquitination
R142 Methylation
K152 Ubiquitination
K162 Ubiquitination
S164 Phosphorylation
S171 Phosphorylation
K175 Ubiquitination
K181 Ubiquitination
K187 Ubiquitination
K193 Ubiquitination
R201 Methylation
K215 Ubiquitination
T252 Phosphorylation
K256 Ubiquitination
K267 Ubiquitination
S270 Phosphorylation
K273 Ubiquitination
S278 Phosphorylation
S282 Phosphorylation
K449 Ubiquitination
Y470 Phosphorylation
K478 Acetylation
K478 Ubiquitination
K479 Ubiquitination
K510 Ubiquitination
S519 Phosphorylation
K528 Ubiquitination
K529 Ubiquitination

Research Backgrounds

Function:

Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

PTMs:

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Subcellular Location:

Cytoplasm. Mitochondrion.
Note: Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Colocalizes with pi- and piP-bodies, a subset of the nuage which contains secondary piRNAs. Associated with mitochondria in the germline.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with (symmetrically methylated) PIWIL1, PIWIL2 and PIWIL4.

Family&Domains:

Belongs to the Tdrkh family.

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