TGM1 Antibody | Affinity Biosciences

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TGM1 Antibody - Western blot analysis of extracts from Mouse kidney tissue, using TGM1 Antibody.

Product:	TGM1 Antibody
Catalog:	DF12770
Description:	Rabbit polyclonal antibody to TGM1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	90 kDa; 90kD(Calculated).
Uniprot:	P22735
RRID:	AB_2845731

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TGM1 Antibody detects endogenous levels of total TGM1.

RRID:

AB_2845731
Cite Format: Affinity Biosciences Cat# DF12770, RRID:AB_2845731.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARCI1; Epidermal TGase; ICR2; KTG; LI; LI1; Protein glutamine gamma glutamyltransferase K; Protein-glutamine gamma-glutamyltransferase K; TG(K); TGase 1; TGASE; TGase K; TGase-1; TGK; TGM1; TGM1_HUMAN; Transglutaminase 1 (K polypeptide epidermal type I, protein glutamine gamma glutamyltransferase); Transglutaminase 1; Transglutaminase K; Transglutaminase, keratinocyte; Transglutaminase-1;

Immunogens

Immunogen:

Uniprot:

P22735(TGM1_HUMAN) >>Visit HPA database.

Gene(ID):

TGM1(7051)

Sequence:

P22735 TGM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMDGPRSDVGRWGGNPLQPPTTPSPEPEPEPDGRSRRGGGRSFWARCCGCCSCRNAADDDWGPEPSDSRGRGSSSGTRRPGSRGSDSRRPVSRGSGVNAAGDGTIREGMLVVNGVDLLSSRSDQNRREHHTDEYEYDELIVRRGQPFHMLLLLSRTYESSDRITLELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVVKASGQNLNLRVHTSPNAIIGKFQFTVRTQSDAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVNVSRVISAMVNSLDDNGVLIGNWSGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAISSNMREDITYLYKHPEGSDAERKAVETAAAHGSKPNVYANRGSAEDVAMQVEAQDAVMGQDLMVSVMLINHSSSRRTVKLHLYLSVTFYTGVSGTIFKETKKEVELAPGASDRVTMPVAYKEYRPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPVTLTNVVFRLEGSGLQRPKILNVGDIGGNETVTLRQSFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPAPGDGGFFSDAGGDSHLGETIPMASRGGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Rabbit

100

Pig

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22735 As Substrate

P22735

Site	PTM Type	Source
S24	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
S82	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
S92	Phosphorylation	Uniprot
S95	Phosphorylation	Uniprot
T104	Phosphorylation	Uniprot
Y134	Phosphorylation	Uniprot
Y136	Phosphorylation	Uniprot
Y267	Phosphorylation	Uniprot
Y312	Phosphorylation	Uniprot
Y365	Phosphorylation	Uniprot
Y370	Phosphorylation	Uniprot
T525	Phosphorylation	Uniprot
K545	Ubiquitination	Uniprot
T559	Phosphorylation	Uniprot
S565	Phosphorylation	Uniprot
Y570	Phosphorylation	Uniprot
S755	Phosphorylation	Uniprot

Research Backgrounds

P22735_TGM1_HUMAN

Function:

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation.

PTMs:

Palmitoylated.

The membrane anchorage region possesses a cluster of five cysteines within which fatty acid(s) may become thioester-linked. It is subject to phorbol ester-stimulated phosphorylation and is hypersensitive to proteolysis, which releases the enzyme in a soluble form.

Tyrosine-phosphorylated.

Subcellular Location:

Membrane>Lipid-anchor.

Subunit Structure:

Interacts with PLAAT4.

Family&Domains:

Belongs to the transglutaminase superfamily. Transglutaminase family.

Restrictive clause

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TGM1 Antibody - #DF12770