Product: TSP50 Antibody
Catalog: DF12785
Description: Rabbit polyclonal antibody to TSP50
Application: WB
Reactivity: Human, Mouse
Prediction: Pig
Mol.Wt.: 50 kDa; 43kD(Calculated).
Uniprot: Q9UI38
RRID: AB_2845746

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%)
Clonality:
Polyclonal
Specificity:
TSP50 Antibody detects endogenous levels of total TSP50.
RRID:
AB_2845746
Cite Format: Affinity Biosciences Cat# DF12785, RRID:AB_2845746.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cancer/testis antigen 20; CT 20; CT20; Probable threonine protease PRSS50; PRSS50; Serine protease 50; Testes specific protease 50; Testis specific protease like protein 50; Testis-specific protease-like protein 50; TSP 50; TSP50_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UI38 TSP50_HUMAN:

Testis specific. Differentially expressed in some breast cancer tissues.

Sequence:
MGRWCQTVARGQRPRTSAPSRAGALLLLLLLLRSAGCWGAGEAPGALSTADPADQSVQCVPKATCPSSRPRLLWQTPTTQTLPSTTMETQFPVSEGKVDPYRSCGFSYEQDPTLRDPEAVARRWPWMVSVRANGTHICAGTIIASQWVLTVAHCLIWRDVIYSVRVGSPWIDQMTQTASDVPVLQVIMHSRYRAQRFWSWVGQANDIGLLKLKQELKYSNYVRPICLPGTDYVLKDHSRCTVTGWGLSKADGMWPQFRTIQEKEVIILNNKECDNFYHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYLVGLVSWGAGCQKSEAPPIYLQVSSYQHWIWDCLNGQALALPAPSRTLLLALPLPLSLLAAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Dog
55
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UI38 As Substrate

Site PTM Type Enzyme
T16 Phosphorylation
S17 Phosphorylation
S20 Phosphorylation

Research Backgrounds

Function:

May be involved in proteolysis through its threonine endopeptidase activity.

Subcellular Location:

Endoplasmic reticulum.
Note: May also localize to cytoplasmic membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis specific. Differentially expressed in some breast cancer tissues.

Family&Domains:

Belongs to the peptidase S1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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