WDR19/IFT144 Antibody - #DF12797
| Product: | WDR19/IFT144 Antibody |
| Catalog: | DF12797 |
| Description: | Rabbit polyclonal antibody to WDR19/IFT144 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Rabbit, Chicken |
| Mol.Wt.: | 152 kDa; 152kD(Calculated). |
| Uniprot: | Q8NEZ3 |
| RRID: | AB_2845758 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12797, RRID:AB_2845758.
Fold/Unfold
DYF 2; DYF2; FLJ23127; IFT144; Intraflagellar transport 144 homolog; KIAA1638; ORF26; Oseg6; PWDMP; WD repeat containing protein 19; WD repeat domain 19; WD repeat membrane protein PWDMP; WD repeat-containing protein 19; Wdr19; WDR19_HUMAN;
Immunogens
Some isoforms are tissue-specific. Highly expressed in the prostate. Lower expression in the cerebellum, pituitary gland, fetal lung, and pancreas. In normal prostate, expressed in both basal and luminal epithelial cells. No expression detected in fibromuscular stromal cells, endothelial cells, or infiltrating lymphocytes. Uniformed expression in prostate adenocarcinoma cells.
- Q8NEZ3 WDR19_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRIFSLLEKTWLGAPIQFAWQKTSGNYLAVTGADYIVKIFDRHGQKRSEINLPGNCVAMDWDKDGDVLAVIAEKSSCIYLWDANTNKTSQLDNGMRDQMSFLLWSKVGSFLAVGTVKGNLLIYNHQTSRKIPVLGKHTKRITCGCWNAENLLALGGEDKMITVSNQEGDTIRQTQVRSEPSNMQFFLMKMDDRTSAAESMISVVLGKKTLFFLNLNEPDNPADLEFQQDFGNIVCYNWYGDGRIMIGFSCGHFVVISTHTGELGQEIFQARNHKDNLTSIAVSQTLNKVATCGDNCIKIQDLVDLKDMYVILNLDEENKGLGTLSWTDDGQLLALSTQRGSLHVFLTKLPILGDACSTRIAYLTSLLEVTVANPVEGELPITVSVDVEPNFVAVGLYHLAVGMNNRAWFYVLGENAVKKLKDMEYLGTVASICLHSDYAAALFEGKVQLHLIESEILDAQEERETRLFPAVDDKCRILCHALTSDFLIYGTDTGVVQYFYIEDWQFVNDYRHPVSVKKIFPDPNGTRLVFIDEKSDGFVYCPVNDATYEIPDFSPTIKGVLWENWPMDKGVFIAYDDDKVYTYVFHKDTIQGAKVILAGSTKVPFAHKPLLLYNGELTCQTQSGKVNNIYLSTHGFLSNLKDTGPDELRPMLAQNLMLKRFSDAWEMCRILNDEAAWNELARACLHHMEVEFAIRVYRRIGNVGIVMSLEQIKGIEDYNLLAGHLAMFTNDYNLAQDLYLASSCPIAALEMRRDLQHWDSALQLAKHLAPDQIPFISKEYAIQLEFAGDYVNALAHYEKGITGDNKEHDEACLAGVAQMSIRMGDIRRGVNQALKHPSRVLKRDCGAILENMKQFSEAAQLYEKGLYYDKAASVYIRSKNWAKVGDLLPHVSSPKIHLQYAKAKEADGRYKEAVVAYENAKQWQSVIRIYLDHLNNPEKAVNIVRETQSLDGAKMVARFFLQLGDYGSAIQFLVMSKCNNEAFTLAQQHNKMEIYADIIGSEDTTNEDYQSIALYFEGEKRYLQAGKFFLLCGQYSRALKHFLKCPSSEDNVAIEMAIETVGQAKDELLTNQLIDHLLGENDGMPKDAKYLFRLYMALKQYREAAQTAIIIAREEQSAGNYRNAHDVLFSMYAELKSQKIKIPSEMATNLMILHSYILVKIHVKNGDHMKGARMLIRVANNISKFPSHIVPILTSTVIECHRAGLKNSAFSFAAMLMRPEYRSKIDAKYKKKIEGMVRRPDISEIEEATTPCPFCKFLLPECELLCPGCKNSIPYCIATGRHMLKDDWTVCPHCDFPALYSELKIMLNTESTCPMCSERLNAAQLKKISDCTQYLRTEEEL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NEZ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y28 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K519 | Ubiquitination | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Uniprot | |
| K836 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K871 | Ubiquitination | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| K912 | Ubiquitination | Uniprot | |
| Y918 | Phosphorylation | Uniprot | |
| K940 | Ubiquitination | Uniprot | |
| K955 | Ubiquitination | Uniprot | |
| Y1230 | Phosphorylation | Uniprot | |
| S1273 | Phosphorylation | Uniprot | |
| Y1276 | Phosphorylation | Uniprot | |
| K1328 | Ubiquitination | Uniprot |
Research Backgrounds
As component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in cilia function and/or assembly. Essential for functional IFT-A assembly and ciliary entry of GPCRs. Associates with the BBSome complex to mediate ciliary transport (By similarity).
Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body. Cell projection>Cilium>Photoreceptor outer segment.
Note: Localizes to photoreceptor connecting cilia, to the base of motile cilia in brain ependymal cells and to the base of and along primary cilia in kidney cells.
Some isoforms are tissue-specific. Highly expressed in the prostate. Lower expression in the cerebellum, pituitary gland, fetal lung, and pancreas. In normal prostate, expressed in both basal and luminal epithelial cells. No expression detected in fibromuscular stromal cells, endothelial cells, or infiltrating lymphocytes. Uniformed expression in prostate adenocarcinoma cells.
Component of the IFT complex A (IFT-A) complex. IFT-A complex is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which is associated with TULP3 and a peripheral subcomplex composed of IFT43:WDR35:TTC21B. Interacts (via C-terminal region) with IFT122 (via C-terminal region). Interacts with BBS1 (By similarity). Interacts with TTC25.
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