Product: ZNF143 Antibody
Catalog: DF12802
Description: Rabbit polyclonal antibody to ZNF143
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 68 kDa; 69kD(Calculated).
Uniprot: P52747
RRID: AB_2845763

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ZNF143 Antibody detects endogenous levels of total ZNF143.
RRID:
AB_2845763
Cite Format: Affinity Biosciences Cat# DF12802, RRID:AB_2845763.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC66276; mStaf; pHZ 1; SBF; Selenocysteine tRNA gene transcription activating factor; SPH binding factor; STAF; Zfp143; zgc:66276; Zinc finger protein 143;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P52747 ZN143_HUMAN:

Expressed in all tissues tested, with the strongest expression in ovary.

Sequence:
MLLAQINRDSQGMTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTAFIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDTISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCEYDGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLAMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKGSQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDGTPITVPAHDAVISSAGTHSVAMVTAEGTEGEQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLVATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Xenopus
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52747 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K71 Ubiquitination
Y87 Phosphorylation
K95 Ubiquitination
K213 Sumoylation
K235 Ubiquitination
Y241 Phosphorylation
K246 Ubiquitination
K255 Ubiquitination
Y267 Phosphorylation
K276 Ubiquitination
T280 Phosphorylation
Y282 Phosphorylation
K285 Sumoylation
K285 Ubiquitination
S286 Phosphorylation
T290 Phosphorylation
T292 Phosphorylation
K295 Acetylation
K306 Ubiquitination
K309 Methylation
K309 Sumoylation
K316 Ubiquitination
T322 Phosphorylation
K328 Ubiquitination
T352 Phosphorylation
Y357 Phosphorylation
K375 Sumoylation
K375 Ubiquitination
T382 Phosphorylation
K385 Ubiquitination
Y387 Phosphorylation
K396 Ubiquitination
K424 Ubiquitination
K436 Ubiquitination
K466 Sumoylation
T633 Phosphorylation

Research Backgrounds

Function:

Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested, with the strongest expression in ovary.

Subunit Structure:

Interacts with CHD8.

Family&Domains:

Belongs to the GLI C2H2-type zinc-finger protein family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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