ADHFE1 Antibody - #DF12810
| Product: | ADHFE1 Antibody |
| Catalog: | DF12810 |
| Description: | Rabbit polyclonal antibody to ADHFE1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 50 kDa; 50kD(Calculated). |
| Uniprot: | Q8IWW8 |
| RRID: | AB_2845771 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12810, RRID:AB_2845771.
Fold/Unfold
ADH8; ADHFE 1; ADHFe1; Alcohol dehydrogenase iron-containing protein 1; Alcohol dehydrogenase, iron containing, 1; Fe containing alcohol dehydrogenase; Fe-containing alcohol dehydrogenase; HOT; HOT_HUMAN; Hydroxyacid oxoacid transhydrogenase, mitochondrial; Hydroxyacid-oxoacid transhydrogenase; mitochondrial;
Immunogens
- Q8IWW8 HOT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAARARVAYLLRQLQRAACQCPTHSHTYSQAPGLSPSGKTTDYAFEMAVSNIRYGAAVTKEVGMDLKNMGAKNVCLMTDKNLSKLPPVQVAMDSLVKNGIPFTVYDNVRVEPTDSSFMEAIEFAQKGAFDAYVAVGGGSTMDTCKAANLYASSPHSDFLDYVSAPIGKGKPVSVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKIGITSRAIKPTLGLIDPLHTLHMPARVVANSGFDVLCHALESYTTLPYHLRSPCPSNPITRPAYQGSNPISDIWAIHALRIVAKYLKRAVRNPDDLEARSHMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKMYKAKDYNVDHPLVPHGLSVVLTSPAVFTFTAQMFPERHLEMAEILGADTRTARIQDAGLVLADTLRKFLFDLDVDDGLAAVGYSKADIPALVKGTLPQERVTKLAPCPQSEEDLAALFEASMKLY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IWW8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K82 | Acetylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| Y334 | Phosphorylation | Uniprot | |
| Y343 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
Mitochondrion.
Only expressed in adult liver.
Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.
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