AHI1 Antibody - #DF12815

(2)  
Product: AHI1 Antibody
Catalog: DF12815
Description: Rabbit polyclonal antibody to AHI1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 137 kDa; 137kD(Calculated).
Uniprot: Q8N157
RRID: AB_2845776

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
AHI1 Antibody detects endogenous levels of total AHI1.
RRID:
AB_2845776
Cite Format: Affinity Biosciences Cat# DF12815, RRID:AB_2845776.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Abelson helper integration site 1; Abelson helper integration site 1 protein homolog; Abelson helper integration site; AHI 1; AHI-1; Ahi1; AHI1_HUMAN; Contatins SH3 and WD40 domains; dJ71N10.1; DKFZp686J1653; FLJ14023; FLJ20069; JBTS3; Jouberin; ORF1; OTTHUMP00000017263; OTTHUMP00000017265; OTTHUMP00000234456; OTTHUMP00000234654; OTTHUMP00000234656;

Immunogens

Immunogen:
Uniprot:

Q8N157(AHI1_HUMAN) >>Visit HPA database.

Gene(ID):
AHI1(54806)
Expression:
Q8N157 AHI1_HUMAN:

Highly expressed in the most primitive normal hematopoietic cells. Expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Expressed in kidney (renal collecting duct cells) (at protein level).

Sequence:
MPTAESEAKVKTKVRFEELLKTHSDLMREKKKLKKKLVRSEENISPDTIRSNLHYMKETTSDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEEDKQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREETDLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKEVPVFSKAETSTLTISGDTVEGEQKKESSVRSVSSDSHQDDEISSMEQSTEDSMQDDTKPKPKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIHRTDRLKSDFMISHPMVKIHVVDEHTGQYVKKDDSGRPVSSYYEKENVDYILPIMTQPYDFKQLKSRLPEWEEQIVFNENFPYLLRGSDESPKVILFFEILDFLSVDEIKNNSEVQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLYYPPTKPRSPLSVVEAFEWWSKCPRNHYPSTLYVTVRGLKVPDCIKPSYRSMMALQEEKGKPVHCERHHESSSVDTEPGLEESKEVIKWKRLPGQACRIPNKHLFSLNAGERGCFCLDFSHNGRILAAACASRDGYPIILYEIPSGRFMRELCGHLNIIYDLSWSKDDHYILTSSSDGTARIWKNEINNTNTFRVLPHPSFVYTAKFHPAVRELVVTGCYDSMIRIWKVEMREDSAILVRQFDVHKSFINSLCFDTEGHHMYSGDCTGVIVVWNTYVKINDLEHSVHHWTINKEIKETEFKGIPISYLEIHPNGKRLLIHTKDSTLRIMDLRILVARKFVGAANYREKIHSTLTPCGTFLFAGSEDGIVYVWNPETGEQVAMYSDLPFKSPIRDISYHPFENMVAFCAFGQNEPILLYIYDFHVAQQEAEMFKRYNGTFPLPGIHQSQDALCTCPKLPHQGSFQIDEFVHTESSSTKMQLVKQRLETVTEVIRSCAAKVNKNLSFTSPPAVSSQQSKLKQSNMLTAQEILHQFGFTQTGIISIERKPCNHQVDTAPTVVALYDYTANRSDELTIHRGDIIRVFFKDNEDWWYGSIGKGQEGYFPANHVASETLYQELPPEIKERSPPLSPEEKTKIEKSPAPQKQSINKNKSQDFRLGSESMTHSEMRKEQSHEDQGHIMDTRMRKNKQAGRKVTLIE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
80
Dog
73
Chicken
70
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N157 As Substrate

Site PTM Type Enzyme
S45 Phosphorylation
T145 Phosphorylation
T180 Phosphorylation
K213 Ubiquitination
K229 Ubiquitination
T258 Phosphorylation
K309 Ubiquitination
S331 Phosphorylation
K380 Ubiquitination
K381 Ubiquitination
K394 Ubiquitination
K411 Ubiquitination
T503 Phosphorylation
S507 Phosphorylation
Y531 Phosphorylation
K538 Ubiquitination
K544 Ubiquitination
K557 Ubiquitination
K582 Acetylation
K582 Ubiquitination
K588 Acetylation
K682 Ubiquitination
S733 Phosphorylation
K791 Ubiquitination
K799 Ubiquitination
S804 Phosphorylation
Y805 Phosphorylation
S822 Phosphorylation
K975 Ubiquitination
K980 Ubiquitination
K999 Ubiquitination
S1002 Phosphorylation
S1005 Phosphorylation
S1010 Phosphorylation
K1015 Ubiquitination
T1071 Phosphorylation
K1095 Ubiquitination
Y1112 Phosphorylation
K1120 Ubiquitination
S1123 Phosphorylation
S1127 Phosphorylation
K1142 Ubiquitination
S1150 Phosphorylation
S1157 Phosphorylation
S1163 Phosphorylation

Research Backgrounds

Function:

Involved in vesicle trafficking and required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in neuronal differentiation. As a positive modulator of classical Wnt signaling, may play a crucial role in ciliary signaling during cerebellum embryonic development.

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium basal body. Cell junction>Adherens junction. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: In the retinal photoreceptor cell layer, localizes at the connecting cilium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in the most primitive normal hematopoietic cells. Expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Expressed in kidney (renal collecting duct cells) (at protein level).

Subunit Structure:

Self-associates. Part of the tectonic-like complex (also named B9 complex). Interacts with MKS1. Interacts with NPHP1; probably as heterodimers and/or AHI1(2):NPHP1(2) heterotetramers. Interacts (via SH3 domain) with the dynamin GTPase DNM2. Interacts with HAP1; probably as AHI1(2):HAP1(2) heterotetramers. Interacts with RAB8A (By similarity). Interacts with CEND1 (By similarity). Interacts with CTNNB1/beta-catenin. Interacts with SPATA7 (By similarity).

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