Product: ARD1B Antibody
Catalog: DF12829
Description: Rabbit polyclonal antibody to ARD1B
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: Q9BSU3
RRID: AB_2845790

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(83%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
ARD1B Antibody detects endogenous levels of total ARD1B.
RRID:
AB_2845790
Cite Format: Affinity Biosciences Cat# DF12829, RRID:AB_2845790.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BSU3 NAA11_HUMAN:

Present in several cell lines, with highest levels in MCF-7 cells (at protein level).

Sequence:
MNIRNAQPDDLMNMQHCNLLCLPENYQMKYYLYHGLSWPQLSYIAEDEDGKIVGYVLAKMEEEPDDVPHGHITSLAVKRSHRRLGLAQKLMDQASRAMIENFNAKYVSLHVRKSNRPALHLYSNTLNFQISEVEPKYYADGEDAYAMKRDLSQMADELRRQMDLKKGGYVVLGSRENQETQGSTLSDSEEACQQKNPATEESGSDSKEPKESVESTNVQDSSESSDSTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Sheep
83
Pig
80
Dog
75
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BSU3 As Substrate

Site PTM Type Enzyme
K89 Ubiquitination
K105 Ubiquitination
Y106 Phosphorylation
K136 Acetylation
Y137 Phosphorylation
Y138 Phosphorylation
Y145 Phosphorylation
K148 Acetylation
K148 Ubiquitination
S186 Phosphorylation

Research Backgrounds

Function:

Displays alpha (N-terminal) acetyltransferase activity. Proposed alternative catalytic subunit of the N-terminal acetyltransferase A (NatA) complex.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in several cell lines, with highest levels in MCF-7 cells (at protein level).

Subunit Structure:

Component of the N-terminal acetyltransferase A (NatA) complex composed of NAA11 and NAA15 (Probable). Interacts with HIF1A.

Family&Domains:

Belongs to the acetyltransferase family. ARD1 subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.