ARHGAP10 Antibody - #DF12830

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Product: ARHGAP10 Antibody
Catalog: DF12830
Description: Rabbit polyclonal antibody to ARHGAP10
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 89 kDa; 217kD(Calculated).
Uniprot: Q5T5U3
RRID: AB_2845791

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(80%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ARHGAP10 Antibody detects endogenous levels of total ARHGAP10.
RRID:
AB_2845791
Cite Format: Affinity Biosciences Cat# DF12830, RRID:AB_2845791.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arhgap21; KIAA1424; RHG21_HUMAN; Rho GTPase-activating protein 10; Rho GTPase-activating protein 21; Rho-type GTPase-activating protein 21;

Immunogens

Immunogen:
Uniprot:

Q5T5U3(RHG21_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP21(57584)
Expression:
Q5T5U3 RHG21_HUMAN:

Widely expressed with higher expression in brain, heart, skeletal muscle and placenta.

Sequence:
MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
90
Sheep
90
Pig
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5T5U3 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
K26 Acetylation
K29 Acetylation
K49 Acetylation
S57 Phosphorylation
S131 Phosphorylation
T232 Phosphorylation
S233 Phosphorylation
T234 Phosphorylation
Y244 Phosphorylation
S253 Phosphorylation
T255 Phosphorylation
S286 Phosphorylation
S319 Phosphorylation
S338 Phosphorylation
K347 Methylation
K347 Ubiquitination
Y351 Phosphorylation
S352 Phosphorylation
S377 Phosphorylation
Y392 Phosphorylation
Y395 Phosphorylation
T423 Phosphorylation
Y425 Phosphorylation
S441 Phosphorylation
T442 Phosphorylation
S443 Phosphorylation
S457 Phosphorylation
S459 Phosphorylation
Y471 Phosphorylation
S475 Phosphorylation
S477 Phosphorylation
S483 Phosphorylation
S487 Phosphorylation
S489 Phosphorylation
T493 Phosphorylation
S552 Phosphorylation
R554 Methylation
S563 Phosphorylation
R575 Methylation
S606 Phosphorylation
R609 Methylation
S612 Phosphorylation
S616 Phosphorylation
S625 Phosphorylation
K627 Acetylation
S659 Phosphorylation
S671 Phosphorylation
K681 Ubiquitination
S682 Phosphorylation
S684 Phosphorylation
S717 Phosphorylation
S743 Phosphorylation
T747 Phosphorylation
S755 Phosphorylation
S857 Phosphorylation
S862 Phosphorylation
S867 Phosphorylation
S879 Phosphorylation
S881 Phosphorylation
Y882 Phosphorylation
Y889 Phosphorylation
S896 Phosphorylation
K898 Methylation
K904 Ubiquitination
S911 Phosphorylation
S918 Phosphorylation
S924 Phosphorylation
S925 Phosphorylation
S926 Phosphorylation
S930 Phosphorylation
S954 Phosphorylation
T980 Phosphorylation
S983 Phosphorylation
K1059 Methylation
T1074 Phosphorylation
S1078 Phosphorylation
S1080 Phosphorylation
S1096 Phosphorylation
S1099 Phosphorylation
S1104 Phosphorylation
S1115 Phosphorylation
S1129 Phosphorylation
T1134 Phosphorylation
T1140 Phosphorylation
T1142 Phosphorylation
T1144 Phosphorylation
S1220 Phosphorylation
K1239 Ubiquitination
Y1240 Phosphorylation
T1375 Phosphorylation
S1378 Phosphorylation
S1383 Phosphorylation
K1392 Ubiquitination
S1396 Phosphorylation
Y1404 Phosphorylation
S1412 Phosphorylation
S1418 Phosphorylation
S1431 Phosphorylation
S1432 Phosphorylation
S1433 Phosphorylation
K1443 Sumoylation
S1492 Phosphorylation
S1497 Phosphorylation
T1498 Phosphorylation
S1504 Phosphorylation
S1509 Phosphorylation
S1514 Phosphorylation
T1516 Phosphorylation
S1518 Phosphorylation
S1527 Phosphorylation
S1530 Phosphorylation
S1562 Phosphorylation
S1566 Phosphorylation
S1605 Phosphorylation
S1624 Phosphorylation
S1628 Phosphorylation
T1635 Phosphorylation
S1637 Phosphorylation
S1639 Phosphorylation
S1669 Phosphorylation
S1672 Phosphorylation
S1675 Phosphorylation
S1680 Phosphorylation
S1684 Phosphorylation
S1687 Phosphorylation
T1702 Phosphorylation
S1708 Phosphorylation
S1713 Phosphorylation
S1717 Phosphorylation
S1742 Phosphorylation
S1745 Phosphorylation
T1748 Phosphorylation
K1762 Methylation
K1764 Methylation
S1797 Phosphorylation
T1803 Phosphorylation
S1843 Phosphorylation
T1860 Phosphorylation
S1861 Phosphorylation
T1862 Phosphorylation

Research Backgrounds

Function:

Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions.

PTMs:

Sumoylated with SUMO2 and SUMO3 in proliferating lymphocytes.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein. Cell junction. Cytoplasmic vesicle membrane>Peripheral membrane protein. Cytoplasm>Cytoskeleton.
Note: Localization to the Golgi is dependent on interaction with GTP-bound ARF1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with higher expression in brain, heart, skeletal muscle and placenta.

Subunit Structure:

Interacts with GTP-bound ARF1 and ARF6. Interacts with CTNNA1.

Restrictive clause

 

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