ARHGAP15 Antibody - #DF12831
(3)
| Product: | ARHGAP15 Antibody |
| Catalog: | DF12831 |
| Description: | Rabbit polyclonal antibody to ARHGAP15 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken |
| Mol.Wt.: | 65 kDa; 55kD(Calculated). |
| Uniprot: | Q53QZ3 |
| RRID: | AB_2845792 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(92%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ARHGAP15 Antibody detects endogenous levels of total ARHGAP15.
RRID:
AB_2845792
Cite Format: Affinity Biosciences Cat# DF12831, RRID:AB_2845792.
Cite Format: Affinity Biosciences Cat# DF12831, RRID:AB_2845792.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ArhGAP15; BM046; RHG15_HUMAN; Rho GTPase activating protein 15; Rho GTPase-activating protein 15; Rho type GTPase activating protein 15; Rho-type GTPase-activating protein 15;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q53QZ3 RHG15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQKSTNSDTSVETLNSTRQGTGAVQMRIKNANSHHDRLSQSKSMILTDVGKVTEPISRHRRNHSQHILKDVIPPLEQLMVEKEGYLQKAKIADGGKKLRKNWSTSWIVLSSRRIEFYKESKQQALSNMKTGHKPESVDLCGAHIEWAKEKSSRKNVFQITTVSGNEFLLQSDIDFIILDWFHAIKNAIDRLPKDSSCPSRNLELFKIQRSSSTELLSHYDSDIKEQKPEHRKSLMFRLHHSASDTSDKNRVKSRLKKFITRRPSLKTLQEKGLIKDQIFGSHLHKVCERENSTVPWFVKQCIEAVEKRGLDVDGIYRVSGNLATIQKLRFIVNQEEKLNLDDSQWEDIHVVTGALKMFFRELPEPLFPYSFFEQFVEAIKKQDNNTRIEAVKSLVQKLPPPNRDTMKVLFGHLTKIVAKASKNLMSTQSLGIVFGPTLLRAENETGNMAIHMVYQNQIAELMLSEYSKIFGSEED
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Dog
100
Chicken
100
Rabbit
100
Bovine
92
Zebrafish
83
Pig
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q53QZ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K415 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Subcellular Location:
Cytoplasm. Membrane>Peripheral membrane protein.
Tissue Specificity:
Expressed in lung, liver and lymphoid cells.
Family&Domains:
The PH domain is required for localization to the membrane.
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