BTN3A1 Antibody | Affinity Biosciences

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BTN3A1 Antibody - Western blot analysis of extracts from Jurkat cells, using BTN3A1 Antibody.

Product:	BTN3A1 Antibody
Catalog:	DF12859
Description:	Rabbit polyclonal antibody to BTN3A1
Application:	WB
Reactivity:	Human
Mol.Wt.:	48 kDa; 58kD(Calculated).
Uniprot:	O00481
RRID:	AB_2845820

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

BTN3A1 Antibody detects endogenous levels of total BTN3A1.

RRID:

AB_2845820
Cite Format: Affinity Biosciences Cat# DF12859, RRID:AB_2845820.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BT3A1_HUMAN; BTN3A1; Butyrophilin subfamily 3 member A1; CD277;

Immunogens

Immunogen:

Uniprot:

O00481(BT3A1_HUMAN) >>Visit HPA database.

Gene(ID):

BTN3A1(11119)

Expression:

O00481 BT3A1_HUMAN:

Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.

Sequence:

O00481 BT3A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKMASFLAFLLLNFRVCLLLLQLLMPHSAQFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSDLHVDVKGYKDGGIHLECRSTGWYPQPQIQWSNNKGENIPTVEAPVVADGVGLYAVAASVIMRGSSGEGVSCTIRSSLLGLEKTASISIADPFFRSAQRWIAALAGTLPVLLLLLGGAGYFLWQQQEEKKTQFRKKKREQELREMAWSTMKQEQSTRVKLLEELRWRSIQYASRGERHSAYNEWKKALFKPADVILDPKTANPILLVSEDQRSVQRAKEPQDLPDNPERFNWHYCVLGCESFISGRHYWEVEVGDRKEWHIGVCSKNVQRKGWVKMTPENGFWTMGLTDGNKYRTLTEPRTNLKLPKPPKKVGVFLDYETGDISFYNAVDGSHIHTFLDVSFSEALYPVFRILTLEPTALTICPA

PTMs - O00481 As Substrate

O00481

Site	PTM Type	Source
K83	Ubiquitination	Uniprot
K108	Ubiquitination	Uniprot
R112	Methylation	Uniprot
N115	N-Glycosylation	Uniprot
K123	Ubiquitination	Uniprot
K155	Ubiquitination	Uniprot
K299	Ubiquitination	Uniprot
S316	Phosphorylation	Uniprot
S327	Phosphorylation	Uniprot
K338	Ubiquitination	Uniprot
K347	Ubiquitination	Uniprot
Y441	Phosphorylation	Uniprot

Research Backgrounds

O00481_BT3A1_HUMAN

Function:

Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Restrictive clause

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For Research Use Only.
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BTN3A1 Antibody - #DF12859