Product: C1orf163 Antibody
Catalog: DF12866
Description: Rabbit polyclonal antibody to C1orf163
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: Q96BR5
RRID: AB_2845827

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(90%)
Clonality:
Polyclonal
Specificity:
C1orf163 Antibody detects endogenous levels of total C1orf163.
RRID:
AB_2845827
Cite Format: Affinity Biosciences Cat# DF12866, RRID:AB_2845827.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAGMVDFQDEEQVKSFLENMEVECNYHCYHEKDPDGCYRLVDYLEGIRKNFDEAAKVLKFNCEENQHSDSCYKLGAYYVTGKGGLTQDLKAAARCFLMACEKPGKKSIAACHNVGLLAHDGQVNEDGQPDLGKARDYYTRACDGGYTSSCFNLSAMFLQGAPGFPKDMDLACKYSMKACDLGHIWACANASRMYKLGDGVDKDEAKAEVLKNRAQQLHKEQQKGVQPLTFG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Dog
90
Bovine
88
Sheep
88
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96BR5 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K56 Ubiquitination
K59 Ubiquitination
S68 Phosphorylation
S70 Phosphorylation
K73 Ubiquitination
Y78 Phosphorylation
K82 Ubiquitination
T86 Phosphorylation
K90 Ubiquitination
K106 Ubiquitination
K133 Ubiquitination
K173 Ubiquitination
S191 Phosphorylation
K195 Ubiquitination
K206 Ubiquitination
K211 Ubiquitination
K223 Ubiquitination

Research Backgrounds

Function:

Required for assembly of mitochondrial respiratory chain complex I and complex IV.

Subcellular Location:

Mitochondrion intermembrane space.
Note: The import in the mitochondrion intermembrane space is mediated by CHCHD4/MIA40.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CHCHD4/MIA40 through transient intermolecular disulfide bonds.

Family&Domains:

Belongs to the hcp beta-lactamase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.